miR-7抑制喉鳞癌Hep-2细胞增殖及迁移的研究

目的 探讨上调microRNA-7（miR-7）表达对喉鳞癌Hep-2细胞增殖与迁移的作用及可能机制。方法 通过脂质体转染miR-7模拟物（miR-7 mimics）及随机序列（miR-Scramble），将喉鳞癌Hep-2细胞分成miR-7组、Scramble组和空白对照（Mock）组，实时定量PCR（qPCR）检测转染48 h后各组细胞中miR-7表达，CCK-8法检测转染48 h后的各组细胞的增殖抑制情况，Transwell试验观察转染24 h后细胞迁移能力，qPCR及Western blotting检测PI3K（p110）、AKT及pAKT 的mRNA和蛋白表达情况。结果 miR-7组Hep-2细胞转染miR-7 mimics后miR-7表达水平明显上调，与其余两组比较差异有统计学意义（P＜0.01）；与Scramble组和Mock组相比， miR-7组Hep-2细胞的增殖抑制率升高，迁移能力降低，且PI3K、AKT及pAKT 的mRNA和蛋白水平均降低（P＜0.05）。结论 上调喉鳞癌Hep-2细胞中miR-7表达能够抑制肿瘤细胞的增殖与迁移，其机制可能与抑制PI3K／Akt信号通路有关。

Inhibition effect of miR-7 on proliferation and metastasis of Hep-2 laryngeal carcinoma cells

Objective To explore the effect of up-regulation of microRNA-7（miR-7） on proliferation and metastasis of Hep-2 laryngeal carcinoma cells and its possible mechanism. Methods The Hep-2 laryngeal carcinoma cells were divided into miR-7 group（Hep-2 cells transfected with miR-7 mimics）， Scramble group（Hep-2 cells transfected with miR-Scramble） and Mock group（Hep-2 cells without transfection）. The quantitive real-time PCR（qPCR） was used to test the expression of miR-7 at 48 h after transfection in 3 groups. The CCK-8 assay was used to test the proliferation of Hep-2 cells in each group at 48 h after transfection. Cell metastasis was determined by transwell test at 48 h after transfection. The mRNA and protein levels of PI3K（p110）， AKT and pAKT were examined by qPCR and Western blotting. Results The expression of miR-7 was significantly up-regulated after transfection with miR-7 mimics. The proliferation and metastasis of Hep-2 laryngeal carcinoma cells was extremely decreased after transfection with miR-7. The results from qPCR and Western blotting showed that the mRNA and protein levels of PI3K（p110）， AKT and pAKT were decreased in miR-7 group compared with other two groups（P＜0.05）. Conclusion Up-regulation of miR-7 can inhibit the proliferation and metastasis ability of Hep-2 laryngeal carcinoma cells with possible mechanism of inhibiting PI3K／AKT signal pathway.