| 同时沉默IGF-1R和EGFR基因对肝癌HepG2细胞株生长抑制的作用机理 |
| |
| 引用本文： | 廖玉华,袁玉林,周绪红.同时沉默IGF-1R和EGFR基因对肝癌HepG2细胞株生长抑制的作用机理[J].武汉大学学报(医学版),2011,32(3):303-307,后插2. |
| |
| 作者姓名： | 廖玉华 袁玉林 周绪红 |
| |
| 作者单位： | 1. 武汉大学医学院解剖学教研室,湖北武汉,430071
2. 武汉大学中南医院耳鼻喉科,湖北武汉,430071 |
| |
| 摘 要： | 目的:同时沉默表皮生长因子受体(EGFR)、胰岛素样生长因子-1受体(IGF-1R)基因,研究其抗瘤疗效及作用机理.方法:分别构建靶向EGFR、IGF-1R及两受体的小分子干扰RNA(siRNA-EGFR、siRNA-IGF-1R、siRNA-EGFR&IGF-1R),构建与任何基因无同源的阴性对照质粒(siRNA-H...
|
| 关 键 词： | 表皮生长因子受体 胰岛素样生长因子-1受体 肝癌 RNA干扰 |
Mechanisms of EGFR and IGF-1R Blockade by siRNA Therapy for Growth Control of Hepatocellular Cancer HepG2 Cells |
| |
| LIAO Yuhua,YUAN Yulin,ZHOU Xuhong.Mechanisms of EGFR and IGF-1R Blockade by siRNA Therapy for Growth Control of Hepatocellular Cancer HepG2 Cells[J].Medical Journal of Wuhan University,2011,32(3):303-307,后插2. |
| |
| Authors: | LIAO Yuhua YUAN Yulin ZHOU Xuhong |
| |
| Institution: | LIAO Yuhua1,YUAN Yulin1,ZHOU Xuhong2 1Dept.of Anatomy & Embryology,School of Medicine,Wuhan University,Wuhan 430071,China2Dept.of Otolaryngology,Zhongnan Hospital of Wuhan University,China |
| |
| Abstract: | Objective: To investigate the antineoplastic effects of inhibiting IGF-1R and EGFR and signaling in hepatocellular cancer(HCC) HepG2 cells by siRNA simultaneous blockade of both of IGF-1R and EGFR.Methods: The growth,cell cycle progression,cellular apoptosis,and the related proteins including bcl-2,cdk2,bax and P21 were analyzed by MTT,flow cytometry,and Western blot.Results: Forty-eight hours after transfection,silencing both of EGFR and IGF-1R inhibited transition of the cells from G1 to S phases,up-regul... |
| |
| Keywords: | Epidermal Growth Factor Receptor Insulin-Like Growth Factor-1 Receptor Hepatocellular Cancer RNAi |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
