| 改良CTAB法提取连钱草基因组DNA |
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| 引用本文： | 鄢嘉,罗娟,王小刚. 改良CTAB法提取连钱草基因组DNA[J]. 武汉大学学报(医学版), 2011, 32(4): 432-435 |
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| 作者姓名： | 鄢嘉 罗娟 王小刚 |
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| 作者单位： | 华中科技大学同济医学院药学院,湖北武汉,430030 |
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| 摘 要： | 目的:建立高质量的连钱草基因组DNA的提取方法.方法:在传统十六烷基三甲基溴化铵(CTAB)法的基础上加以改良,比较不同浓度CTAB对提取的连钱草基因组DNA质量的影响,并对所得的DNA采用紫外分光光度计、电泳和PCR扩增等方法进行检测.结果:用2%浓度的CTAB处理所获得的基因组DNA质量较好,蛋白质残留少,RNA消...
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| 关 键 词： | 连钱草 基因组DNA 改良CTAB法 |
A Modified CTAB Method for Extraction of Genome DNA from Glechoma Longituba |
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| YAN Jia,LUO Juan,WANG Xiaogang. A Modified CTAB Method for Extraction of Genome DNA from Glechoma Longituba[J]. Medical Journal of Wuhan University, 2011, 32(4): 432-435 |
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| Authors: | YAN Jia LUO Juan WANG Xiaogang |
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| Affiliation: | YAN Jia,LUO Juan,WANG Xiaogang Tongji College of Pharmacy,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective:To improve the method of extraction high-quality genomic DNA from Glechoma longituba.Methods:The different concentrations of CTAB extraction buffer were adopted for the genomic DNA extraction.The total DNA was detected by UV scanning,electrophoresis and PCR amplification.Results:The genomic DNA extracted with 2% concentration of CTAB is in high-purity and good integrity.DNA was not degraded significantly and RNA was completely digested.PCR fragments show clear and good polymorphism.Conclusion:The ... |
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| Keywords: | Glechoma Longituba Genome DNA Improved CTAB Method |
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