转化生长因子β_1、白细胞介素-1β、白细胞介素-6在豚鼠分泌性中耳炎动物模型中的表达

目的:检测转化生长因子β1(TGF-β1)、白细胞介素-1β(IL-1β),白细胞介素-6(IL-6)在分泌性中耳炎(SOM)动物模型鼓室黏膜中的表达,探讨TGF-β1、IL-1β、IL-6在SOM发病和转归中的作用。方法:应用灭活肺炎链球菌注入豚鼠中耳腔,制作SOM动物模型,采用免疫组织化学方法检测致病鼠鼓室黏膜上皮在注入细菌后6h、1 d、3 d、7 d、14 d及30 d后TGF-β1,IL-1β,IL-6的表达。结果:第3天造模成功,SOM动物模型的鼓室黏膜上皮中TGF-β1、IL-1β,IL-6急性期均有表达。IL-1β术后3 d达高峰,IL-6术后1 d达高峰,TGF-β1在术后7 d表达明显增强,第30天时达高峰。结论:灭活肺炎链球菌致SOM中,IL-1β,IL-6在SOM早期发挥作用,TGF-β1可能与SOM慢性迁延有关。

Expression of Transforming Growth Factor Beta 1,Interleukin 1 Beta,and Interleukin-6 in the Middle Ear Mucosa of Guinea Pigs with Secretory Otitis Media

Objective: To explore the expression of transforming growth factor β1(TGF-β1),interleukin 1β(IL-1β),and interleukin-6(IL-6) in the mucosa of guinea pigs with secretory otitis media(SOM) and the role of TGF-β1,IL-1β,and IL-6 in the development of SOM.Methods: SOM was produced by injecting deactivated streptococcus pneumoniae into the tympanum cavity of guinea pigs.The expression of TGF-β1,IL-1β,and IL-6 was examined by immunohistochemistry in 6 hours,and 1,3,7,14 and 30 day(s) after injection.Results: The expression of TGF-β1,IL-1β,and IL-6 could be early detected in the middle ear mucosa.Interleukin 1β could be detected at the 6th hour after injection and reached the peak level on the 3rd day.The expression of TGF-β1 began to increase on the 7th day after injection and reached the peak level on the 30th day.Conclusion: The expression manners of TGF-β1,IL-1β,and IL-6 are different in SOM induced by deactivated streptococcus pneumoniae,and this study indicates that TGF-β1 may play a role in the chronic protraction of SOM,while IL-1β and IL-6 may play a different role in the early stage of SOM.