| SELEX技术筛选特异性结合高转移性肺癌细胞Anip973的单链DNA适配子 |
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| 引用本文： | 李金明,章志怀,彭振元.SELEX技术筛选特异性结合高转移性肺癌细胞Anip973的单链DNA适配子[J].武汉大学学报(医学版),2011,32(3):329-333. |
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| 作者姓名： | 李金明 章志怀 彭振元 |
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| 作者单位： | 1. 湖北省黄冈市黄州区妇女儿童医院外科,湖北黄冈,438000
2. 湖北省黄冈市中心医院外科,湖北黄冈,438000 |
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| 摘 要： | 目的:筛选获得一种高亲和力的能特异性结合高转移性肺癌细胞Anip973的单链DNA适配子.方法:体外合成全长88 nt的单链DNA文库,通过反复的SELEX筛选获得特异性结合Anip973细胞的单链DNA适配子,并通过生物素-辣根过氧化物酶-链霉亲和素系统初步测定筛选的适配子与细胞的亲和力,将结合力最高的适配子库进一步...
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| 关 键 词： | SELEX技术 肺癌细胞 DNA适配子 |
In Vitro Selection of ssDNA Aptamers Against Lung Cancer Cells by SELEX |
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| LI Jinming,ZHANG Zhihuai,PENG Zhenyuan.In Vitro Selection of ssDNA Aptamers Against Lung Cancer Cells by SELEX[J].Medical Journal of Wuhan University,2011,32(3):329-333. |
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| Authors: | LI Jinming ZHANG Zhihuai PENG Zhenyuan |
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| Abstract: | Objective: To screen ssDNA aptamers that can specifically bind to the lung cancer cell line Anip973.Methods: A single strand DNA library with 88nt in length containing a random sequence of 30nt was synthesized in vitro.The aptamers that against the Anip973 cells were obtained by SELEX after multiple screening.The affinity between the aptamers and Anip973 cells was determined by Biotin-HRP-Streptavidin system and the ssDNA which has highest affinity was cloned and sequenced.Results: After 11 rounds positive ... |
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| Keywords: | SELEX Lung Cancer Cells DNA Aptamer |
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