白细胞介素-6反义核苷酸抑制胆管癌细胞增殖和诱导其凋亡的实验研究

目的:研究白细胞介素-6(IL-6)反义核苷酸(ASODN)对人肝内胆管癌HCCC-9810细胞增殖和凋亡的影响.方法:通过脂质体Lipofectamine"2000介导IL-6 ASODN转染人肝内胆管癌HCCC-9810细胞.细胞克隆率形成实验和MTT实验检测IL-6 ASODN对胆管癌细胞增殖的影响,流式细胞仪检...

Effect of Interleukin-6 Antisenseoligodeoxynucleotides on Proliferation and Apoptosis of Human Bile Duct Carcinoma Cellline

Objective: To investigate the effect of interleukin-6 (IL-6) antisense oligonucleotide (ASODN) on proliferation and apoptosis of human bileductcarcinoma cell line HCCC-9810. Methods: IL-6 ASODN was transfected into HCCC-9810 cells by LipofectamineTM 2000. Cell colony efficiency experiment and MTT were adopted to determine HCCC-9810 cells’ proliferation. The apoptosis was analyzed by flow cytometry. The expression of IL-6 mRNA was detected by RT-PCR. Results: After transfection, the cell clone forming rate in the ASODN transfected cells was (4.58±0.35)%, which was significantly lower than that in the control cells (P<0.05). The cell proliferation inhibition rate in the ASODN transfected cells was 67.2%, which was significantly higher than that in the control cells (P<0.05). The apoptosis rate in the ASODN transfected cells was (10.25±0.5) %, which was significantly higher in the ASODN transfected cells than that in the control cells (P<0.05). The IL-6 mRNA was decreased by 60.52% in the ASODN transfected cells as compared with that in the blank group, and was significantly lower than that in the control cells (P<0.05). Conclusion: ASODN targeting at IL-6 can inhibit HCCC-9810 cells’ proliferation and induce apoptosis.