| 人结肠癌细胞HT-29血管生成能力与微缺氧的关系 |
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| 引用本文： | 杨庆强,唐春燕.人结肠癌细胞HT-29血管生成能力与微缺氧的关系[J].西部医学,2011,23(8):1427-1430. |
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| 作者姓名： | 杨庆强 唐春燕 |
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| 作者单位： | 1. 泸州医学院附属医院普外科,四川,泸州,646000
2. 泸州医学院护理学院 |
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| 摘 要： | 目的观察HT-29细胞在微缺氧下的血管生成能力,检测OPN的表达,以探讨其向恶性表型转化的潜在机制。方法参照体内肿瘤的氧分压,建立微缺氧模型。内皮细胞小管形成试验检测体外促进新生血管形成的能力。将HT-29细胞微缺氧处理0、6、12、24、48小时,RT-PCR和Western blot检测OPN的mRNA和蛋白表达。结果微缺氧组内皮细胞形成的管状结构数为（28.5±3.6）,明显多于对照组（12.4±2.8）,差异具有统计学意义（P〈0.01）。微缺氧6h,HT-29细胞OPN的mRNA和蛋白无明显变化,随着微缺氧时间的延长,其表达逐渐上调,24h达顶峰,48h与24h无显著差异。结论微缺氧能诱导HT-29细胞促进新生血管形成,而促进其向恶性表型转化。微缺氧下OPN普遍上调,OPN可能是微缺氧下继HIF-1α之外的另一重要调控因子,该调控因子与微缺氧诱导的恶性表型密切相关。
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| 关 键 词： | 微缺氧 骨桥蛋白 恶性表型 |
Moderate hypoxia up-regulates the expression of osteopontin and augment angiogenesis in colon carcinoma cell line HT-29 |
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| YANG Qing-qiang.Moderate hypoxia up-regulates the expression of osteopontin and augment angiogenesis in colon carcinoma cell line HT-29[J].Medical Journal of West China,2011,23(8):1427-1430. |
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| Authors: | YANG Qing-qiang |
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| Institution: | YANG Qing-qiang(Department of General Surgery,The Affiliated Hospital of Luzhou Medical College,Luzhou Medical College,Luzhou 646000,Sichuan) |
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| Abstract: | Objective To investigate correlation between moderate hypoxia and tumor angiogenesis in colon carcinoma cell line HT-29,detect the expression of osteopontin and explore its latent mechanism of maliglant transformation.Methods The moderate hypoxia model was established in accordance with the PO2 of tumor in vivo.Tube formation of human umbilical vein endothelial cells(HUVECs) were adopted to evaluate the ability of tumor angiogenesis in vitro.0,6,12,24 or 48h after moderate hypoxia,osteopontin mRNA and prote... |
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| Keywords: | Moderate hypoxia Osteopontin Malignant phenotype |
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