自身免疫性前列腺炎对大鼠前列腺形态学与炎性基因表达的影响

目的:运用Wistar大鼠前列腺蛋白提纯液辅以弗氏完全佐剂,建立自身免疫性慢性前列腺炎模型,观察前列腺形态学与炎性基因表达情况。方法:以Wistar大鼠为实验动物,0、30d腹腔注射百白破疫苗,皮内多点注射前列腺蛋白提纯液辅及弗氏完全佐剂,60d观察大鼠前列腺组织的大体、光镜、电镜病理形态学,以及运用实时荧光定量PCR法,观察炎性基因表达产物的变化。结果:实验组炎性基因表达产物促嗜酸粒细胞聚集趋化因子,iNOS,血清白细胞介素4显著升高,光镜、电镜表现出慢性炎症病理改变。结论:慢性前列腺炎可能是一种自身免疫性疾病。

Morphological and Proinflammatory Expression of the Experimental Autoimmune Prostatitis in the Rat Model

Objective:To establish a Wistar rat model of chronic abacterial prostatitis(CAP)by injecting purified prostate protein and Freund's complete adjuvant,and to study the influence on the morphology and proinflammatory expression.Methods:Male rats were injected with the Pertussis-Diptheria-Tetanus vaccine into the abdominal cavity and purified prostate protein and Freund's complete adjuvant intradermally at 0 and 30 days.At 60 days,the rats were sacrificed,and then the prostate specimens were observed,under the light microscope and electron microscope,and the changes of proinflummatory expression was observed too,using PCR technique.Results:The products of proinflammatory expression,such as eotaxin,iNOS and IL-4 increased markedly.The change of chronic inflammation was shown by light microscope and electron microscope.Conclusion:Chronic prostatitis is associated with autoimmunity.