| 芍药甙对PC12细胞缺血性损伤的保护及机制研究 |
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| 引用本文： | 魏守建,杨军,何丽娜,何素冰.芍药甙对PC12细胞缺血性损伤的保护及机制研究[J].中国中医急症,2000,9(6):274-276. |
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| 作者姓名： | 魏守建 杨军 何丽娜 何素冰 |
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| 作者单位： | 1. 安徽中医学院临床医院一系中药教研室,合肥,230038
2. 安徽省医学科学研究所省生物医药重点实验室,合肥,230061 |
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| 摘 要： | 目的 探讨芍药甙（ＰＡＥ）对ＰＣ１２细胞缺血样损伤模型的保护作用及其机制。方法 采用组织培养法，以肾上腺嗜铬瘤克隆化细胞株ＰＣ１２细胞为材料，制备缺糖损伤、缺氧损伤、自由基损伤Ｃａｆ损伤、ＮＯ损伤及ＧＩｕ毒性损伤模型。结果 形态学检查发现ＰＡＥ对６种脑缺血样损伤模型中ＰＣ１２细胞具有明显保护作用，ＭＴＴ染色提示ＰＡＥ可显著提高损伤模型中ＰＣ１２细胞的存活数，降低胞浆释放的ＬＤＨ水平。结论 除缺氧损
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| 关 键 词： | 芍药甙 PC12细胞 脑缺血样损伤 钙超载 |
| 文章编号： | 1004-745X(2000)06-0274-03 |
| 修稿时间： | 2000年6月18日 |
Protective Effect of Paeoniflorin Against Ischemic Injury in Cultured PC12 Cells |
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| Wei Shoujian,Yang jun,He Lina,et al..Protective Effect of Paeoniflorin Against Ischemic Injury in Cultured PC12 Cells[J].Journal of Emergency in Traditional Chinese Medicine,2000,9(6):274-276. |
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| Authors: | Wei Shoujian Yang jun He Lina |
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| Abstract: | To investigate protective effect and mechanism of paeoniflorin(PAE)on ischemia- like injury models. Methods:Six ischemic injury models including hypoxia,hypoglucose,oxidant injury,calcium overload,nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti- ischemic effect of PAE in cultured PC12 cells. Results:The morphological examination founded that PAE had obvious protective effect on PC12 cells in above six models.MTT staining and LDH measurement also indicated that PAE significantly increased number of live cells and lessened the extent of cell injury. Conclusion:PAE protected PC12 cells from ischemic injury effectively in vitro.The mechanism of its action may focus on blocking calcium channel of cell membrane. |
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| Keywords: | PAE PC12 cells Ischemic injury Calcium overload |
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