趋化因子CCL20在慢性乙型肝炎活检组织中的表达及意义

目的 研究慢性乙型肝炎(CHB)肝活检组织CCL20的表达与病理分级分期的关系,探讨CCL20在慢性HBV感染中的作用及意义.方法 通过内参照竞争逆转录聚合酶链反应(RT-PCR)对处于正常状态和慢性感染状态的肝细胞系的CC120 mRNA进行定量检测,观察CCL20的表达差异.定量检测正常肝脏和CHB活检组织的CC120水平,结合肝脏组织学积分研究其相关性.结果 在体外细胞系水平上,HBV未感染肝细胞HepG2的CCL20表达量为(2.65±0.02)pg/106细胞,而HBV持续感染肝细胞HepG2.2.15的CC120表达量为(1.22±0.04)pg/106细胞(t=39.66,P<0.01);PMA刺激CCL20表达的增加但不改变CCL20在二类细胞之间的表达格局.在HBV慢性感染状态下肝活检组织的CCL20的表达(3.54±0.65)pg/20 mg显著低于其在正常肝组织中的平均表达量(8.74±0.56)pg/20 mg(t=30.09,P<0.01),且与肝脏的组织学积分相关(r=0.675,P=0.023).结论 HBV慢性感染状态下CCL20的表达下调,CCL20的表达与CHB患者肝脏组织学积分相关.

Significance of CCL20 expression in liver biopsies of chronic hepatitis B patients

Objective To observe the correlation of histologicalactivity(HAI) of chronic hepatitis B (CHB) with CCL20 expression, and to investigate the impact of CCL20 expression in CHB infection. Methods On the basis of established competitive quantitative RT-PCR with an internal standard, the expression of the CCL20 in the hepatocytes in different infected patterns of HBV infected cells and liver biopsies were quantified and at the same time its correlation to HAI were explored. Results In the cell levels, the expression quantity of CCL20 in control cells (HepG2), persistent HBV infected hepatocytes( HepG2. 2. 15) are (2. 65 ± 0. 02) pg/106 cells, ( 1.22± 0. 04) pg/106 cells, respectively. There were significantly differences between them ( t = 39. 66, P < 0. 01 ). The expression of CCL20 was enhanced in hepatocytes stimulated by PMA but their expression pauern was not changed. Moreover, CCL20 expression in liver biopsies with CHB was (3.54 ± 0. 65 ) pg/20 mg and CCL20 expression in control groups was ( 8. 74±0. 56) pg/20 mg. The expression of CCL20 between two groups was different (t =30. 09,P <0. 01 ) and correlation lied in between HAI and CCL20 expression in liver biopsies of CHB patients ( r = 0. 675, P =0. 023 ). Conclusion CCL20 expression was down-regulated and it was correlated to HAI of liver biopsies in CHB patients.