反相微乳液法制备麦醇蛋白纳米颗粒的初步研究

目的 常规去溶剂法制备的麦醇蛋白颗粒因粒径偏大（400 ～ 500 nm）易被网状内皮系统捕获。采用反相微乳液法制备粒径 〈 100 nm的麦醇蛋白纳米颗粒。方法 用反相微乳法，以液体石蜡为油相，二硫苏糖醇（DTT）还原的麦醇蛋白乙醇溶液为水相，Tween80-Span80为复合乳化剂。按质量比1/7 ~ 1/3称取Tween80和Span80，按质量比1/6 ~ 1/2与石蜡混合均匀，配制复合乳化剂和油相的混合溶液。在水相液滴中，麦醇蛋白被加入的H2O2氧化形成二硫键而交联固化，形成纳米颗粒。结果 乳液制备工艺与复合乳化剂配比、复合乳化剂与油相质量比、搅拌速度有关，得到最佳制备工艺为：以液体石蜡为油相，5.0 mg/mL麦醇蛋白乙醇/水（70 %，v/v）溶液为水相，Tween80-Span80（1/7，w/w）为复合乳化剂，乙醇为助乳化剂，水相/油相比例 2/30（v/w），复合乳化剂/油相比例 1/3（w/w） ，搅拌速度1 000 r/min。得到麦醇蛋白纳米颗粒粒径为20 ～ 100 nm，颗粒中不含DTT、复合乳化剂和H2O2，纳米颗粒的热稳定性优于麦醇蛋白原料，并且纳米颗粒无细胞毒性，且细胞毒性评级均为0级。结论 用反相微乳法可以获得用作为药物载体的麦醇蛋白纳米颗粒。 更多

Preliminary study on the preparation of gliadin nanoparticles with reverse microemulsion method

Objective To prepare gliadin nanoparticles（NPs） with sizes less than 100 nm by gliadin reverse microemulsion, because traditional gliadin particles prepared with desolation method are likely to be trapped by retieulo-endothelial system due to their large sizes（400 - 500 nm）. Methods Gliadin NPs with smaller sizes were prepared by gliadin reverse microemulsion, the disculfide bond （DqT） reduced gliadin in ethanol-water solution, liquid paraffin, and Tween80-Spau80 were served as water phase（W）, oil phase（O） and emulsifier, respectively. Compound emulsifier and oil phase mixture solution was prepared by mixing Tween80 and Span80 with mass ratio of 1/7 - 1/3 and mixed with wax with mass ratio of 1/6 - 1/2. In aqueous solution, gliadin was cross-linked due to the oxidized disulfide generated by treating with H202, formed NPs. Results Emulsion preparation were related with composite emulsifiers, emulsifier and oil phase ratio, and stirring speed. The optimal preparation conditions were 5.0 mg/mL gliadin solution in W phase, 1 ： 7（w/w） of Tween80 ： Span80, 2/30（v/w） of W phase/O phase, 1/3（w/w） of Tween80-Span80/O phase and 1 000 r/rain of stirring speed. The results showed that the sizes of NPs ranged from 20 - 100 nm. DTI＇, emulsifiers and H202 were removed completely from NPs gliadiu. NPs were more thermally stable than gliadin protein. Additionally, the cytotoxicity of gliadin NPs was level 0. Conclusion It is demonstrated that gliadin NPs are successfully prepared with reverse microemulsion method, and could serve as an alternative for drug delivery.