| 紫外诱变和氨基酸抗性筛选选育那西肽高产菌株 |
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| 引用本文： | 秦艳飞,薛正莲,项驷文,李恒奎,王洲.紫外诱变和氨基酸抗性筛选选育那西肽高产菌株[J].中国抗生素杂志,2011,36(8). |
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| 作者姓名： | 秦艳飞 薛正莲 项驷文 李恒奎 王洲 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,微生物发酵安徼省工程技术研究中心,芜湖,241000 |
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| 基金项目： | 安徽工程大学青年基金项目 |
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| 摘 要： | 目的 筛选出那西肽高产菌株.方法 以活跃链霉菌CB040517作为出发菌株,通过紫外-氧化锂诱变处理,并结合前体复合氨基酸抗性筛选,选育那西肽高产菌.结果 通过致死率和正突变率的考察,确定紫外最佳诱变剂量为100s.在分离培养基上层加入氯化锂和底层加入复合氨基酸进行正突变株的定向筛选,选育得到那西肽高产菌株UV-19-A12,其摇瓶效价达904.00μg/mL,比出发菌株提高72.5%,经过五代斜面传代试验考察,该菌株遗传性状稳定.结论 紫外诱变和氨基酸抗性筛选可以获得那西肽高产菌株.
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| 关 键 词： | 活跃链霉菌 那西肽 紫外诱变 氨基酸抗性筛选 |
Breeding high-yielding strains of nosiheptide with UV mutagenesis and amino acids resistance screening |
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| Qin Yan-fei,Xue Zheng-lian,Xiang Si-wen,Li Heng-kui,Wang Zhou.Breeding high-yielding strains of nosiheptide with UV mutagenesis and amino acids resistance screening[J].Chinese Journal of Antibiotics,2011,36(8). |
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| Authors: | Qin Yan-fei Xue Zheng-lian Xiang Si-wen Li Heng-kui Wang Zhou |
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| Institution: | Qin Yan-fei,Xue Zheng-lian,Xiang Si-wen,Li Heng-kui and Wang Zhou(Institute of Biologic & Chemical Engineering of Anhui Polytechnic University,Microorganism Fermentation Engineering and Technology Research Center of Anhui Province,Wuhu 241000) |
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| Abstract: | Objective To screen out Streptomyces actuosu strains of high yield of nosiheptide.Methods UV mutagenesis and prosoma compound amino acids were used to screen high-yielding strains of nosiheptide directionly by using Streptomyces actuosu CB040517 as orginal strain.Results The results showed that the dose of UV irradiation was determined 100s in term of death rate and positive mutation.Using isolation medium with lithium chloride on the upper layer and compound amino acids on the bottom layer,a high-yield nos... |
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| Keywords: | Streptomyces actuosu Nosiheptide UV mutagenesis Amino acids resistance screening |
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