| 高良姜素对PC12细胞氧化损伤的保护作用及其机制研究 |
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| 引用本文： | 陈晓素,杨晓凯,林一均,杨晓国,张旭.高良姜素对PC12细胞氧化损伤的保护作用及其机制研究[J].上海中医药大学学报,2016,30(3):93-96. |
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| 作者姓名： | 陈晓素 杨晓凯 林一均 杨晓国 张旭 |
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| 作者单位： | 温州医科大学温州市第三临床学院温州市人民医院神经内科浙江温州325000,温州医科大学温州市第三临床学院温州市人民医院神经内科浙江温州325000,温州医科大学温州市第三临床学院温州市人民医院神经内科浙江温州325000,温州医科大学温州市第三临床学院温州市人民医院神经内科浙江温州325000,温州医科大学附属第一医院浙江温州325000 |
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| 摘 要： | 目的:探讨高良姜素对H_2O_2诱导的PC12细胞氧化损伤的保护作用及其作用机制。方法:用H_2O_2损伤PC12细胞建立氧化应激损伤模型,细胞分为正常对照组、模型对照组、高良姜素(低、中、高浓度)组,并给予相应干预措施。采用MTT法检测细胞生长抑制率,采用Annexin-V-FITC凋亡检测试剂盒检测细胞凋亡情况,免疫印迹法检测蛋白表达情况。结果:高良姜素预处理可以减少H_2O_2诱导的PC12细胞氧化应激损伤;高良姜素能明显抑制细胞凋亡,且呈剂量依赖性;高良姜素可以增加PI3K/Akt和Bcl-2家族蛋白比例。结论:高良姜素是通过激活PI3K/Akt信号通路发挥对H_2O_2诱导的PC12细胞氧化损伤的保护作用。
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| 关 键 词： | 高良姜素 氧化损伤 细胞凋亡 PC12细胞 |
| 收稿时间： | 2016/1/12 0:00:00 |
Protective Effect and Its Mechanisms of Galangin on Cytotoxicity in PC12 Cells Induced by H2O2 |
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| CHEN Xiao-su,YANG Xiao-kai,LIN Yi-jun,YANG Xiao-guo and ZHANG Xu.Protective Effect and Its Mechanisms of Galangin on Cytotoxicity in PC12 Cells Induced by H2O2[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2016,30(3):93-96. |
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| Authors: | CHEN Xiao-su YANG Xiao-kai LIN Yi-jun YANG Xiao-guo and ZHANG Xu |
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| Institution: | Department of Neurology, The Third Clinical Hospital of Whenzhou City, Wenzhou Medical University,Whenzhou, Zhejiang, 325000,Department of Neurology, The Third Clinical Hospital of Whenzhou City, Wenzhou Medical University,Whenzhou, Zhejiang, 325000,Department of Neurology, The Third Clinical Hospital of Whenzhou City, Wenzhou Medical University,Whenzhou, Zhejiang, 325000,Department of Neurology, The Third Clinical Hospital of Whenzhou City, Wenzhou Medical University,Whenzhou, Zhejiang, 325000 and Department of Neurology,The First Affiliated Hospital of Wenzhou Medical University, Whenzhou, Zhejiang, 325000 |
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| Abstract: | Objective:To study the protective effect and its possible mechanisms of galangin on H2O2-induced cytotoxicity in PC 12 cell. Methods: A model of oxidative stress in PC12 cells injury was established by H2O2.Cells were divided into control group, model group, galangin(low-, medium-, high-dosage)groups. The viability of PC12 cells was assessed by MTT assay, cell apoptosis was detected by Annexin-V-FITC Apoptosis Detection Kit, and Western blotting method was carried out to detect the protein expressions. Results: Pretreatment with galangin could reduce H2O2-induced oxidative stress; galangin significantly inhibited cell apoptosis with a dose-dependent manner; and galangin could increase the PI3K/Akt and Bcl-2 family prote in ratios in cells. Conclusion: Galangin can protect H2O2-induced PC 12 cell injury by activating PI3K/Akt pathway. |
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| Keywords: | galangin oxidative stress injury apoptosis PC12 cell |
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