| 端粒酶逆转录酶在皮肤肿瘤原位表达水平的比较 |
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| 引用本文： | 丁颖果,武玲慎,涂平. 端粒酶逆转录酶在皮肤肿瘤原位表达水平的比较[J]. 中国皮肤性病学杂志, 2004, 18(1): 8-10 |
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| 作者姓名： | 丁颖果 武玲慎 涂平 |
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| 作者单位： | 北京大学第一医院皮肤性病科,北京,100034 |
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| 摘 要： | 目的 研究端粒酶在皮肤恶性肿瘤发病机制中的作用。方法 采用人端粒酶逆转录酶 (hTERT)cRNA探针与石蜡标本进行原位杂交的方法检测 3 0例皮肤基底细胞癌、15例皮肤鳞状细胞癌、19例脂溢性角化、14例正常皮肤中hTERTmRNA的表达水平 ,并进行比较。结果 hTERT阳性率基底细胞癌为 73 .3 5 %(2 2 /3 0 ) ,鳞状细胞癌为80 .0 0 %(12 /15 ) ,均明显高于脂溢性角化 3 6.84%(7/19)和正常皮肤 2 8.5 7%(4 /14 ) ,并具有统计学意义。结论 hTERT在恶性皮肤肿瘤中的阳性表达率明显高于良性肿瘤和正常皮肤 ,提示端粒酶在皮肤恶性肿瘤发病机制中起着重要作用。原位杂交检测hTERT表达水平的方法有可能成为鉴别皮肤良恶性肿瘤的辅助检查手段。
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| 关 键 词： | 皮肤肿瘤 端粒酶 端粒酶逆转录酶,人 原位杂交 |
| 文章编号： | 1001-7089(2004)01-0008-03 |
In Situ Hybridization Analysis of the Expression of Human Telomerase Reverse Transcriptase(hTERT) in Skin Tumors |
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| DING Ying-guo,WU Ling-shen,TU Ping. In Situ Hybridization Analysis of the Expression of Human Telomerase Reverse Transcriptase(hTERT) in Skin Tumors[J]. The Chinese Journal of Dermatovenereology, 2004, 18(1): 8-10 |
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| Authors: | DING Ying-guo WU Ling-shen TU Ping |
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| Abstract: | Objective To study the role of telomerase in the pathogenesis of malignant skin tumors.Methods Digoxiginin-labled hTERT cRNA probe was applied to in situ hybridize to mRNA of hTERT in paraffin-embedded sections of 30 cases of basal cell carcinoma,15 cases of squamous cell carcinoma,19 cases of seborrheic keratosis and 15 cases of normal skin.Results In 22 of 30 cases of basal cell carcinoma samples and 12 of 15 cases of squamous cell carcinoma samples, hTERT was expressed at a higher level than that of seborrheic keratosis samples(7 positives of 19)and normal skin samples(4 positives of 14).Conclusion Telomerase may play an important role in the pathogenesis of malignant skin tumors.hTERT may be used as a marker for the differentiation between malignant and benign skin tumors. |
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