神经生长因子对拟AD模型大鼠脑内神经原纤维缠结的影响

目的: 研究神经生长因子对拟阿尔茨海默病(Alzheimer's Disease,AD)模型大鼠海马及皮层中神经原纤维缠结(NFT)的影响.方法: 采用冈田酸(Okadaic Acid,OA)海马CA1区微量多次注射建立拟AD大鼠模型,侧脑室注射神经生长因子(nerve growth factor, NGF)进行预处理,观察大鼠行为学变化,Bielschowsky染色观察海马及皮层神经原纤维缠结(neurobrillary tangles,NFT).结果: OA注射后,大鼠出现认知能力、学习记忆能力减退,海马及皮层出现较多NFT;注射NGF预处理后,上述症状改善,海马及皮层NFT数量减少.结论: NGF可显著改善拟AD大鼠模型学习记忆能力,表明NGF可抑制NFT的形成,改善AD病的症状.

The Effect of Nerve Growth Factor on Neurofibrillary Tangles in Alzheimer's Disease-like Model Rat Brain

Objective: To investigate the effect of nerve growth factor(NGF) on neurofibrillary tangles(NFT) in hippocampal and cortex region of Alzheimer's disease(AD)-like model rats.Methods: Okadaic acid(OA) was injected into hippocamal CA1 region of rats with microdosis repeatedly to establish Alzheimer's disease-like model.NGF was injected into lateral cerebral ventricle as pretreatment.The behavior change of rats was observed.NFT in hippocampus and cortex was observed on Bielschowsky stained sections.Results: After injection of OA,the learning impairment and the memory deficits of rats was shown,and more NFT were appeared in hippocamal and cortex.After NGF pretreatment,symptoms mentioned above were remitted,and NFT amounts were reduced in hippocamal and cortex.Conclusions: NGF can impove the learning and memory capability of the model rats,inhibit the formation of NFT,and remit AD symptoms.