乙交酯-丙交酯共聚物支架-细胞复合体移植对大鼠损伤脊髓的修复作用

目的 观察神经干细胞、雪旺细胞和组织工程材料乙交酯-丙交酯共聚物共移植后对大鼠损伤脊髓形态和功能的修复作用.方法 36只Wistar大鼠,随机数字法分为乙交酯-丙交酯共聚物移植组、神经干细胞/乙交酯-丙交酯共聚物绀和神经干细胞+雪旺细胞/乙交酯-丙交酯共聚物组.体外培养、鉴定胚胎脊髓源神经干细胞和雪旺细胞,制备和构建乙交酯-丙交酯共聚物支架细胞复合体并移植到大鼠脊髓T9半横断损伤部位,应用BBB行为评分和电生理技术在术后4、12周评价大鼠脊髓功能的恢复情况;应用透射电镜、HE染色和免疫组织化学染色方法在形态结构上观察轴突和髓鞘再生情况,以及神经干细胞在脊髓内的存活、迁移和分化情况.结果术后4、12周,细胞移植组的BBB评分较对照组明显提高(P＜0.05);细胞移植组的体感诱发电位和运动诱发电位波幅较对照组都有所好转.术后12周移植材料正中横断面透射电镜可见新生的无髓及有髓神经纤维;脊髓标本免疫组织化学染色显示移植的神经十细胞呵以在宿主脊髓内存活、迁移并分化成神经元和少枝胶质细胞,未分化成星形胶质细胞.结论 神经干细胞、雪旺细胞和组织工程材料乙交酯-丙交酯共聚物共移植可以促进半横断损伤的大鼠脊髓轴突再生,改善肢体的运动功能.

Co-transplantation of neural stem cell and Schwann cell and a PLGA scaffold to repair spinal cord injury in rats

Objective To observe the morphological and functional repairing effects of co-transplantation of neural stem cell (NSC), Schwann cell (SC) and a polylactide-co-glycolide acid (PLGA) scaffold on the spinal cord injury (SCI) in rats. Methods Thirty-six wistar rats were divided into 3 equal groups randomly according to the transplantation materials: PLGA (A), NSC/PLGA (B)and NSC+SC/PLGA(C).The complex of PLGA scaffold/cells Was constructed and implanted into the injury site of T9 in SCI models developed from wistar rats. BBB locomotion scores, motor evoked potentials (MEP) and somatosensory evoked potentials (SEP) of lower limbs were examined to learn the rehabilitation of sensory and motor funotions at 4 and 12 weeks after injury in difierent groups. Al the SCI tissues were observed with transelectronmicroscopy. HE and immunohistochemistry to identify the regeneration of neural fibers and the survival, migration and differentiation of the NSC at 4 and 12 weeks after injury. Results From 4 to 12 weeks, the BBB locomotion scores in all the transplantation groups increased, especially in the CO-transplantation groups(P＜0.05).The amplitudes of SEP and MEP at 4 weeks became lower than at 12 weeks after injury in groups B and C, but they were not observed at 4 and 12 weeks after injury in group A. The regenerated axons were observed in the scaffold/cells complex with transelectronmicroseopy. Immunohistochemistry staining showed the transplanted NSCs could survive. Migrate and difierentiate into neuron and oligodendrocyte at 12 weeks. Conclusion Co-transplantation of NSC+SC/PLGA can promote the functional recovery of the injured spinal cord.