| 特苄康唑对真菌麦角甾醇生物合成的影响 |
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| 引用本文： | 曹永兵,姜远英,殷明,杨鹏远,张万年. 特苄康唑对真菌麦角甾醇生物合成的影响[J]. 中国药学杂志, 2000, 35(4): 237-240 |
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| 作者姓名： | 曹永兵 姜远英 殷明 杨鹏远 张万年 |
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| 作者单位： | 1. 上海 200433 第二军医大学药学院药理学教研室
2. 药物化学教研室 |
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| 基金项目： | 国家自然科学基金资助项目!第39870869号，军队“九五”医药科研规划重点课题!第96Z03 0号，上海市科技发展基金资助项目!第98QB |
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| 摘 要： | 目的：研究新化合物特苄康唑对真菌麦角甾醇生物合成的影响。方法：用薄层色说谱语扫描法考察真菌经不同浓度特苄康唑作用后，其麦角甾醇和羊毛甾醇的含量变化。结果：特苄康唑能使真菌中的麦角甾醇含量下降，羊毛甾醇含童增加，两者的含量变化均有明显的剂量依赖性；同浓度时特苄康唑对真菌生长繁殖和麦角甾醇生物合成的抑制作用无显著差异。结论：特苄康唑是通过抑制真菌的麦角甾醇生物合成而发挥其高效抗真菌活性的，为羊毛甾醇14a-去甲基化酶抑制剂。
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| 关 键 词： | 特苄康唑 羊毛甾醇14a-去甲基化酶 麦角甾醇 羊毛甾醇 |
| 收稿时间： | 1999-09-17; |
Effect of terbiaminazole a new antifungal agent,on ergosterol biosynthesis in fungi |
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| Cao Yongbing. Effect of terbiaminazole a new antifungal agent,on ergosterol biosynthesis in fungi[J]. Chinese Pharmaceutical Journal, 2000, 35(4): 237-240 |
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| Authors: | Cao Yongbing |
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| Affiliation: | (Cao YB),Jiang Yuanying (Jiang YY),Yin Ming (Yin M |
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| Abstract: | OBJECTIVE: To study the effect of terbiaminazole on ergosterol biosynthesis in fungi. METHODS: Saponification and extraction of the nonsaponifiable lipids (NSLs) of fungi were carried out after 24 h of incubation with varied concentrations of terbiaminazole.Eggosterol and lanosterol in the NSLs were separated and their concentrations were determined by the method of thin layer chromatography (TLC) scanning. RESULTS: Terbiaminazole inhibited ergosterol biosynthesis and caused the concentration of lanosterol increased with a dose dependent manner in fungi.The inhibitory rates of terbiaminazole on ergosterol biosynthesis and fungi growth were not different significantly (P＞0.05). CONCLUSION: Terbiaminazole,which has antifungal activity because of its inhibition on ergosterol biosynthesis in fungi,is a inhibitor of lanosterol 14 α-demethlylase. |
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| Keywords: | terbiaminazole lanosterol 14 α-demethlylase ergosterol lanosterol |
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