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| 羧基-D-氨基葡萄糖对人食管癌Eca-109细胞caspase-3活化及bcl-2蛋白表达的影响 | |
| 引用本文： | 吴静,强占荣,林香春,杨国栋,周永宁,王爱勤,薛群基. 羧基-D-氨基葡萄糖对人食管癌Eca-109细胞caspase-3活化及bcl-2蛋白表达的影响[J]. 第三军医大学学报, 2009, 31(6) |
| 作者姓名： | 吴静 强占荣 林香春 杨国栋 周永宁 王爱勤 薛群基 |
| 作者单位： | 1. 北京世纪坛医院,北京大学第九临床医学院消化科,北京100038;兰州大学第一医院消化科,兰州730000 2. 兰州大学第一医院消化科,兰州,730000 3. 北京世纪坛医院,北京大学第九临床医学院消化科,北京100038 4. 中国科学院兰州化学物理研究所,兰州,730000 |
| 基金项目： | 中国科学院"西部之光"资助项目 |
| 摘 要： | 目的 探讨D-氨基葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(COPADG)对人食管癌Eca-109细胞凋亡的影响及其作用机制.方法 体外培养Eca-109细胞;倒置显微镜下观察细胞形态学变化;流式细胞术检测COPADG作用后Eca-109细胞内半胱氨酸酶3(caspase-3)活化程度及bcl-2蛋白表达的变化;Annexin V/PI染色结合流式细胞术检测COPADG作用后Eca-109细胞凋亡率.结果 COPADG作用显著增加Eca-109细胞凋亡率,且Eca-109细胞内caspase-3活性和bcl-2表达平均荧光强度明显增高.结论 COPADG能显著诱导Eca-109细胞凋亡发生,并使Eca-109细胞内caspase-3显著活化以及bcl-2蛋白表达下调,并可能通过该机制诱导细胞凋亡的发生. |
| 关 键 词： | 2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖 食管癌 凋亡 半胱氨酸酶3 |
Effect of 2-((3-carboxy-1-oxopropyl)amino)-2-deoxy-D-glucose on Caspase-3 activation and Bcl-2 expression in human esophageal cancer cell line Eca-109 |
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| WU Jing,QIANG Zhan-rong,LIN Xiang-chun,YANG Guo-dong,ZHOU Yong-ning,WANG Ai-qin,XUE Qun-ji. Effect of 2-((3-carboxy-1-oxopropyl)amino)-2-deoxy-D-glucose on Caspase-3 activation and Bcl-2 expression in human esophageal cancer cell line Eca-109[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(6) | |
| Authors: | WU Jing QIANG Zhan-rong LIN Xiang-chun YANG Guo-dong ZHOU Yong-ning WANG Ai-qin XUE Qun-ji |
| Affiliation: | WU Jing1,2,QIANG Zhan-rong2,LIN Xiang-chun1,YANG Guo-dong2,ZHOU Yong-ning2,WANG Ai-qin3,XUE Qun-ji3 (1Department of Gastroenterology,Ninth Clinic Medicine College of Beijing University,Beijing Shijitan Hospital,Beijing 100038,2Department of Gastroenterology,First Hospital of Lanzhou University,Lanzhou 730000,3Lanzhou Institute of Chemical and Physics,Chinese Academy of Sciences,China) |
| Abstract: | Objective To observe the effect of D-glucosamine 2-((3-carboxy-1-oxopropyl)amino)-2-deoxy-D-glucose (COPADG) on apoptosis and caspase-3 activation and bcl-2 protein expression in human esophageal cancer Eca-109 cells and discuss the possible mechanisms. Methods Eca-109 cells were cultured in vitro, then exposured to 0.03 mmol/L COPADG for different time periods. The cell morphological changes were observed by inverted phase contrast microscopy. Caspase-3 activity and bcl-2 expression were detected by flow c... |
| Keywords: | bcl-2 |
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