低砷染毒大鼠肾脏蛋白质组学研究

目的对低剂量砷暴露大鼠肾脏进行蛋白质组学研究,探讨早期砷暴露的生物学标志物。方法选取健康雄性SD大鼠48只,按体质量随机分为4组,每组12只,即对照组(0μg/L),低剂量染毒组(50μg/L),中剂量染毒组(150μg/L),高剂量染毒组(450μg/L)。染毒4周后处死大鼠,取其肾脏运用蛋白质组学技术(双向凝胶电泳)分离蛋白,凝胶经扫描后用Pdquest图形分析软件对2-DE图谱进行了分析,鉴定染毒SD大鼠和正常SD大鼠肾脏蛋白质的差异表达。结果定性分析发现,与对照组比较,低剂量组新出现了2个蛋白质点,消失了4个蛋白质点;中剂量组新出现了4个蛋白质点,消失了3个蛋白质点;高剂量组新出现了3个蛋白质点,消失了7个蛋白质点。定量分析发现,与对照组比较,低剂量组表达量升高的蛋白质点有4个,降低的有4个;中剂量组表达量升高的蛋白质点有11个,降低的有2个;高剂量组表达量升高的蛋白质点有3个,降低的有4个。结论低砷染毒导致大鼠肾组织中蛋白质差异表达,这些蛋白质可能与砷暴露的毒性作用有关。

Renal proteomic analysis of low-concentration arsenic exposure in rats

Objective To investigate the early-phase biomarkers of low-concentration arsenic exposure on the kidneys in rats by proteomie analysis. Methods Forty-eight rats were randomly allocated to be treated with arsenic at the concentration of 0 μg/L （blank control group, n=12）, 50 μg/L （low-concentration exposure group, n=12）, 150 μg/L （moderate-concentration exposure group, n=12） and 450 μg/L （high-concentration exposure group, n=12）, respectively. At week 4, all rats were sacrificed for harvesting the kidneys. This was followed by segregation of the target protein by two-dimensional gel electrophoresis （2-DE） that entailed analysis on the scanned plot by using Pdquest, an image processing software, to determine the differential expression in control group and intoxicated rats. Results Compared with control group, qualitative analysis evidenced 2 emerging and 4 vanished spots in the low-concentration exposure group. And these figures were 4 and 3 in moderate-concentration exposure group and 3 and 7 in high-concentration exposure group, respectively. Quantitative analysis suggested that, compared with control group, there were 4 respec- tive up- and down-regulated spots in low-concentration exposure group. And these figures were 11 and 1 in moderate- concentration exposure group and 3 and 4 in high-concentration exposure group, respectively. Conclusion The fact that low-concentration arsenic exposure results in differential protein expression of the kidney in rats may be linked to the toxicity of arsenic exposure.