| 缺糖在PC12细胞中诱发的细胞毒性及bcl-2基因的表达 |
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| 引用本文： | 宋晓冬,田伟,魏冠英,刘炎,刘雯,左伋. 缺糖在PC12细胞中诱发的细胞毒性及bcl-2基因的表达[J]. 解剖学杂志, 2006, 29(3): 275-278,281 |
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| 作者姓名： | 宋晓冬 田伟 魏冠英 刘炎 刘雯 左伋 |
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| 作者单位： | 滨州医学院生物学教研室,滨州,256603;复旦大学细胞与遗传医学教研室,上海,200032 |
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| 摘 要： | 目的:研究PC12细胞缺糖损伤的分子生物学特征。方法:建立细胞缺糖模型,电镜观测PC12细胞形态;SOD法检测超氧化物歧化酶活性;流式细胞仪检测细胞凋亡率;RT-PCR测定bcl-2、bcl-xl基因表达。结果:缺糖细胞有凋亡现象发生;细胞缺糖24 h后,培养液内的SOD和细胞内的SOD活力明显降低;bcl-2、bcl-xl基因在6~16 h表达上调,此后表达下调。结论:缺糖损伤的PC12细胞既有形态的变化也有功能的变化,细胞膜受到自由基的攻击,细胞抗氧化能力下降,细胞同时伴随着凋亡和坏死两种死亡方式,bcl-2、bcl-xl基因的表达具有一定的时序和范围特点。
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| 关 键 词： | 缺糖 PC12细胞 凋亡 bcl-2 bcl-xl 基因表达 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005-08-222005-10-25 |
Glucose deprivation induced cytotoxicity and bcl-2 expression in PC12 cell line |
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| SONG Xiao-Dong,TIAN Wei,WEI Guan-Ying,LIU Yan,LIU Wen,ZUO Ji. Glucose deprivation induced cytotoxicity and bcl-2 expression in PC12 cell line[J]. Chinese Journal of Anatomy, 2006, 29(3): 275-278,281 |
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| Authors: | SONG Xiao-Dong TIAN Wei WEI Guan-Ying LIU Yan LIU Wen ZUO Ji |
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| Affiliation: | 1 .Department of Biology, Binzhou Medical College, Binzhou 256603, China ;2 .Department of Cellular and Genetic Medicine, Fudan University, Shanghai 200032, China |
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| Abstract: | Objective:To probe into the pathological mechanism of the injury induced by glucose deprivation(an insult relevant to the brain ischemia) in PC12 cell line,providing a basis for studying the mechaism for post-eschemia neuron damage.Methods: Electron microscopy,SOD measurement,Flow Cytometry and RT-PCR were used.Results: The apoptosis and necrosis were observed in the treated PC12 cells,with significant decrease of SOD values.Bcl2 and bcl-xl RNA expression upregulated from 6 to 16 hours after glucose deprivation treatment and declined gradually since then.Conclusion: The PC12 cells deprived of glucose have the morphological and functional alterations including apoptosis and necrosis. |
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| Keywords: | glucose deprivation PC12 cell apoptosis bcl-2 bcl-xl gene expression |
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