端粒重复扩增技术测定端粒酶在不同肿瘤细胞中的分布

目的：确定端粒酶在几种恶性肿瘤细胞和肿瘤组织中的分布状态及其临床意义。方法：采用端粒重复扩增技术（Telomere Repeat Amplifecation Protocol,TRAP）测定人鼻咽癌细胞KB、新鲜乳癌组织细胞、乳癌转移淋巴结细胞、人宫颈癌细胞系Hela、小鼠腹水癌细胞P388、人白血病细胞K652及人肺癌细胞A549中端粒酶的表达水平。结果：测试显示，新鲜乳癌组织细胞、人宫颈癌细胞Hela、小鼠淋巴细胞白血病细胞系P388及人肺癌细胞系A549均出现端粒酶的阳性表达，阳性率为50%。结论：证实了端粒酶在一些恶性肿瘤细胞组织中的存在，同时也反映了端粒酶与上述肿瘤细胞的恶性增殖行为的密切联系。

Detection of the telomere distribution in various cancer cells by telomere repeat amplification protocol

Objective To identify the clincial meanings of the telomere activity distribution in various cancer cells.Methods RT-PCR with telomere repeat amplification protocol (TRAP) was applied to express telomere in different cancer cells in breast cancer tissue cells,cancer cells in lymphonode metastasis from breast cancer,nasopharynx carcinoma KB,carcinoma of the uterine cervix Hela,mouse peritoneal carcinoma P388,leukemia cell line K562 and Lung cancer cell line A549.Results The telomere activity was found in human of breast cancer tissue cells,carcinoma of the uterine cervix Hela,mouse peritoneal carcinoma P388,and Lung cancer cell line A549.Conclusion The telomerase is expressed in various malignant tumor tissues and cells,and related closely with the malignant biological behavior of the tumor cells above.