| 浙江汉族人群KIR3DL2等位基因的多态性分析 |
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| 引用本文： | 姜侃,朱发明,章伟,吕沁风,何吉,严力行.浙江汉族人群KIR3DL2等位基因的多态性分析[J].中华医学遗传学杂志,2006,23(3):339-342. |
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| 作者姓名： | 姜侃 朱发明 章伟 吕沁风 何吉 严力行 |
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| 作者单位： | 1. 310006,杭州,浙江省血液中心,卫生部血液安全重点研究实验室;浙江大学医学院附属第一医院
2. 310006,杭州,浙江省血液中心,卫生部血液安全重点研究实验室 |
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| 基金项目： | 浙江省自然科学基金（Y204216）;浙江省医药卫生科学研究基金（2004A014） |
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| 摘 要： | 目的探讨浙江汉族人群KIR3DL2等位基因的多态性分布情况。方法KIR3DL2等位基因分型采用聚合酶链反应寡核苷酸探针杂交方法，利用引物特异性扩增KIR3DL2基因的两个片段，合成21条探针检测KIR3DL2基因的多态性位点，根据探针的格局判断出样本的KIR3DL2等位基因情况。结果样本中共发现17种探针格局，检测到7种等位基因，其中KIR3DL2＊002等位基因占57％。1例样本无法用现有等位基因探针格局进行分析。结论聚合酶链反应寡核苷酸探针杂交方法可有效检测KIR3DL2等位基因，浙江汉族人群KIR3DL2等位基因的分布有其特点。
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| 关 键 词： | 杀伤细胞免疫球蛋白样受体 基因频率 聚合酶链反应寡核苷酸探针 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005年8月20日 |
Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population |
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| JIANG Kan,ZHU Fa-ming,ZHANG Wei,LU Qin-feng,HE Ji,YAN Li-xing.Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population[J].Chinese Journal of Medical Genetics,2006,23(3):339-342. |
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| Authors: | JIANG Kan ZHU Fa-ming ZHANG Wei LU Qin-feng HE Ji YAN Li-xing |
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| Institution: | Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Minstry of Health, Hangzhou, Zhejiang, 310006 PR China. |
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| Abstract: | Objective To analyze the allelic polymorphism of killer cell immunoglobulin (Ig)-like receptor (KIR) gene 3DL2 in Zhejiang Han population. Methods Allelic definition within KIR3DL2 was achieved through using the PCR-sequence specific oligonucleotide probes (PCR-SSOP) method and the specific amplification of exon 3-exon 4 and exon 8-exon 9. Twenty-one digoxigenin-labelled probes were used to the SSOP analysis. Results Seven alleles of KIR3DL2 were observed, in which KIR3DL2*002 had the highest allele frequency, 0.57. One sample could not be given the allele combination according to the probe profile. Conclusion The PCR-SSOP method was reliable. There is a distinctive allele frequency of KIR3DL2 in Zhejiang Han population. |
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| Keywords: | killer cell immunnglobulin-like receptor gene frequency polymerase chain reaction-sequence specific oligonucleotide probes |
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