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| GAT-1下调对氧化槐定碱镇痛作用的影响 | |
| 引用本文： | 高进贤,易正洪,杨光,闫琳,陶丽君,高栓生,潘秋燕,蒋袁絮. GAT-1下调对氧化槐定碱镇痛作用的影响[J]. 中国中药杂志, 2011, 36(23): 3315-3318 |
| 作者姓名： | 高进贤 易正洪 杨光 闫琳 陶丽君 高栓生 潘秋燕 蒋袁絮 |
| 作者单位： | 1. 甘肃省人民医院药剂科,甘肃兰州,730000 2. 宁夏医科大学基础医学院药理学系宁夏回药现代化工程技术研究中心,宁夏银川,750004 3. 宁夏自治区人民医院药剂科,宁夏银川,750004 |
| 基金项目： | 国家自然科学基金项目(30860372) |
| 摘 要： | 目的:研究中枢GAT-1下调对氧化槐定碱(oxysophoridine,OSR)镇痛作用的影响.方法:采用热板法观察并分析γ-氨基丁酸(gamma aminobutyric acid,GABA)及GABA运载蛋白(GABA transporter 1,GAT-1)抑制剂NO-711与OSR镇痛的协同作用；采用Real time RT-PCR方法检测OSR对福尔马林致痛小鼠脊髓和大脑GAT-1 mRNA表达的影响,分析中枢GAT-1的变化对OSR镇痛作用的影响.结果:GABA(2.0 mg·kg-1,icv)和NO-711(0.125 mg·kg-1,icv)均可显著加强阈下镇痛剂量OSR(32.0 mg·kg-1,iv)在小鼠热板反应中的镇痛作用,使小鼠痛阈明显提高(P ＜0.05,P＜0.01)；OSR(500.0 mg·kg-1,iv)可明显抑制福尔马林致痛小鼠脊髓和大脑GAT-1 mRNA的表达(P＜0.05,P＜0.01).结论:中枢GAT-1参与了OSR的镇痛作用,GAT-1基因下调能增强OSR的镇痛作用. |
| 关 键 词： | 氧化槐定碱 镇痛 GAT-1 脊髓 大脑 |
| 收稿时间： | 2011-01-21 |
Effect of down-regulation of GAT-1 on analgesic action of oxysophoridine |
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| GAO Jinxian,YI Zhenhong,YANG Guang,Yan Lin,TAO Lijun,GAO Shuansheng,PAN Qiuyan and JIANG Yuanxu. Effect of down-regulation of GAT-1 on analgesic action of oxysophoridine[J]. China Journal of Chinese Materia Medica, 2011, 36(23): 3315-3318 | |
| Authors: | GAO Jinxian YI Zhenhong YANG Guang Yan Lin TAO Lijun GAO Shuansheng PAN Qiuyan JIANG Yuanxu |
| Affiliation: | Department of Pharmacy, People's Hospital of Gansu Province, Gansu 730000, China;Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine,Yinchuan 750004, China;Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine,Yinchuan 750004, China;Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine,Yinchuan 750004, China;Department of Pharmacy, Ningxia People's Hospital, Yinchuan 750004, China;Department of Pharmacy, People's Hospital of Gansu Province, Gansu 730000, China;Department of Pharmacy, People's Hospital of Gansu Province, Gansu 730000, China;Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine,Yinchuan 750004, China |
| Abstract: | Objective: To study the effect of GABA transporter(GAT-1)on the analgesic action of oxysophoridine(OSR) in the central nervous system of mice. Method: Hot plate test was used to observe and analyze the effect of gamma aminobutyric acid and the inhibitor of GAT-1 (NO-711) on the analgesic action of oxysophoridine. Real time RT-PCR was used to investigate the influence of OSR on the expression of GAT-1 mRNA induced by formalin in spinal cord and brain of mice. Result: Both GABA (2.0 mg·kg-1,icv) and NO-711(0.125 mg·kg-1,icv) enhanced the analgesic action of OSR (32.0 mg·kg-1,iv) in the hot plate test, and the latencies was markedly increased (P<0.05,P<0.01). OSR (500.0 mg·kg-1,iv) significantly inhibited the expression of GAT-1 mRNA induced by formalin(P<0.05). Conclusion: GAT-1 was involved in the analgesia effect of OSR and the down-regulation of GAT-1 mRNA enhanced the analgesic effect. |
| Keywords: | oxysophoridine analgesia GAT-1 spinal cord brain |
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