| 补阳还五汤指纹图谱总量统计矩加合性的研究 |
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| 引用本文： | 段晓鹏,贺福元,周晋,曾姣丽,谢相贵,王海琴. 补阳还五汤指纹图谱总量统计矩加合性的研究[J]. 中国中药杂志, 2011, 36(23): 3247-3252 |
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| 作者姓名： | 段晓鹏 贺福元 周晋 曾姣丽 谢相贵 王海琴 |
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| 作者单位： | 1. 湖南中医药大学药学院,湖南长沙,410208
2. 湖南中医药大学药学院,湖南长沙410208;湖南中医药大学中药药性与药效国家中医药管理局重点实验室,湖南长沙410208;湖南中医药大学现代中药制剂制备技术与评价实验室,湖南长沙410208 |
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| 基金项目： | 国家自然科学基金项目(81073142,81173558) |
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| 摘 要： | 目的:采用总量统计矩法分析不同溶剂处理所获得的补阳还五汤HPLC指纹图谱,验证总量统计矩法的加合性.方法:采用水提醇沉法获取补阳还五汤总浸出物,依次用溶度参数1 1.4 ～23.40 Cal1/2·cm-3/2的5种溶剂溶解制样；测定各溶剂浸出物的HPLC指纹图谱,计算并分析其总量统计矩参数的叠加规律.结果:以补阳还五汤总方液指纹图谱为参照峰,用正丁醇(11.4 Cal1/2·cm-3/2)、甲醇( 13.5 Cal1/2·cm-3/2)、68％甲醇(16.67 Cal1/2·cm-3/2)、34％甲醇(20.03 Cal1/2·cm -/2)、水(23.40 Cal1/2·cm -3/2)依次溶解所获得的浸出物指纹图谱的相似度分别为0.074,0.973,0.934,0.991,0.993,其总量零阶矩、一阶矩、二阶矩的RSD为63.04％,16.22％,69.38％,表明各指纹图谱差异性较大；按指纹图谱加合性公式计算的总量零阶矩、一阶矩、二阶矩分别为3.203×105 mAu·s,29.85 min,389.97 min2,而实际测得的总方浸出物指纹图谱的总量零阶矩、一阶矩、二阶矩分别为6.548×104mAu·g,29.44 min,389.00 min2；两者极差绝对值百分率分别为2.209％,1.389％,0.248 4％.结论:中药指纹图谱总量统计矩法具有加合性,可用于中药多成分体系静态与动态的质量监控分析.
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| 关 键 词： | 补阳还五汤 指纹图谱 总量统计矩 加合性 溶度参数 |
| 收稿时间： | 2011-05-21 |
Study of additive properties of total quantum statistical moment of chromatographic fingerprint for Buyang Huanwu decoction |
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| DUAN Xiaopeng,HE Fuyuan,ZHOU Jin,ZENG Jiaoli,XIE Xianggui and WANG Haiqin. Study of additive properties of total quantum statistical moment of chromatographic fingerprint for Buyang Huanwu decoction[J]. China Journal of Chinese Materia Medica, 2011, 36(23): 3247-3252 |
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| Authors: | DUAN Xiaopeng HE Fuyuan ZHOU Jin ZENG Jiaoli XIE Xianggui WANG Haiqin |
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| Affiliation: | The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China; Property and Efficacy Key Laboratory of Traditional Chinese Materia Medica, State Administration of Traditional Chinese Medicine, Changsha 410208, China; Pharmaceutical Preparation Technology and Evaluation Laboratory of Traditional Chinese Medicine, Hunan University of Tradition Chinese Medicine, Changsha 410208, China;The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;The Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China |
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| Abstract: | |
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| Keywords: | Buyang Huanwu decoction chromatographic fingerprint total quantum statistical moment additive properties solubility parameter |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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