| 响应面法优化车前子油的提取工艺及抗氧化活性研究 |
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| 引用本文： | 徐伟,凌伟坚,褚克丹,李煌,陈立典,张玉琴,陈娴雯.响应面法优化车前子油的提取工艺及抗氧化活性研究[J].中国中药杂志,2011,36(23):3253-3257. |
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| 作者姓名： | 徐伟 凌伟坚 褚克丹 李煌 陈立典 张玉琴 陈娴雯 |
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| 作者单位： | 1. 福建中医药大学药学院,福建福州,350108
2. 福建中医药大学康复医学院,福建福州,350108 |
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| 基金项目： | 福建省科技厅重大专项(2009YZ0001-1-1);福建省科技厅科技创新平台项目(2010Y2004) |
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| 摘 要： | 目的:优化超临界CO2萃取车前子油的最佳工艺条件,研究车前子油中脂肪酸的组成成分和含量以及其抗氧化活性.方法:在单因素实验基础上,利用响应面法,以萃取率为响应值,建立数学模型,获得最佳工艺；利用GC-MS分析车前子油中脂肪酸组成及含量,并采用自由基清除能力体系初步评价车前子油的抗氧化活性.结果:通过二次回归模型响应面分析得出最佳超临界CO2萃取车前子油最佳工艺条件是萃取温度70℃,萃取压力30 MPa,萃取时间120 min,CO2流速30 L·h-1,在此最佳工艺条件下车前子油萃取率为35.07％,理论值为35.91％,与理论值相对误差小于5％.经GC-MS分析得车前子油主要成分为亚麻酸、亚油酸、硬脂酸和棕榈酸,其中不饱和脂肪酸含量超过88％.抗氧化活性研究中,由DPPH+和ABTS+自由基清除实验得IC50分别为1.13,3.57g·L-1.结论:由响应面法优化得到的车前子的提取工艺方便可行,得到的车前子油具有较强的抗氧化活性,值得进一步开发应用.
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| 关 键 词： | 车前子油 超临界CO2萃取法 响应面法 GC-MS 抗氧化活性 |
| 收稿时间： | 8/2/2011 12:00:00 AM |
Optimization of supercritical CO2 extraction of Plantaginis Semen oil by response surface methodology and studies on its antioxidant activity |
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| XU Wei,LING Weijian,CHU Kedan,LI Huang,CHEN Lidian,ZHANG Yuqin and CHEN Xianwen.Optimization of supercritical CO2 extraction of Plantaginis Semen oil by response surface methodology and studies on its antioxidant activity[J].China Journal of Chinese Materia Medica,2011,36(23):3253-3257. |
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| Authors: | XU Wei LING Weijian CHU Kedan LI Huang CHEN Lidian ZHANG Yuqin and CHEN Xianwen |
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| Institution: | Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Convalescence Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China |
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| Abstract: | Objective:The optimum conditions of SFE-CO2 extraction of Plantaginis Semen oil(SPO),the composition of SPO and its antioxidant activities of SPO were all investigated in this paper.Method:Response surface method(RSM) was used to establish the mathematical model of SFE-CO2 extraction of SPO to obtain the optimum conditions based on Single factor experiments.Fatty acid compositions and contents of SPO were tested by GC-MS,and antioxidant activities of SPO were studied by DPPH and ABTS free radical eliminatio... |
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| Keywords: | Plantaginis Semen oil supercritical fluid extraction response surface methodology fatty acids antioxidant activities |
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