| 流式荧光法检测抗MICB抗体的建立及初步应用 |
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| 引用本文： | 樊一笋,曾万杰,耿春松,胡振华,陈强,丁双双. 流式荧光法检测抗MICB抗体的建立及初步应用[J]. 临床检验杂志, 2011, 29(8): 567-569 |
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| 作者姓名： | 樊一笋 曾万杰 耿春松 胡振华 陈强 丁双双 |
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| 作者单位： | 上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021;上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021;上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021;上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021;上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021;上海交通大学医学院苏州九龙医院检验科，江苏苏州 215021 |
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| 摘 要： | 目的 建立稳定、快速和特异的抗MHC-I类链相关基因B(MICB)抗体流式荧光检测方法,了解MICB抗体在健康人群中的分布频率.方法 克隆、表达和分离纯化MICB融合蛋白,将其包被在Luminex微球表面,建立MICB抗体流式荧光检测方法.评价方法的线性范围、特异性、精密度、准确性,并用此方法检测500例健康人血清MI...
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| 关 键 词： | MICB 流式荧光 等位基因多态性 抗体 |
| 收稿时间： | 2011-08-15 |
| 修稿时间： | 2011-09-22 |
Development and application of the method for detection of anti-MICB antibodies by using Luminex flow cytometry |
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| FAN Yi-sun,ZENG Wan-jie,GENG Chun-song,HU Zhen-hua,CHEN Qiang,DING Shuang-shuang. Development and application of the method for detection of anti-MICB antibodies by using Luminex flow cytometry[J]. Chinese Journal of Clinical Laboratory Science, 2011, 29(8): 567-569 |
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| Authors: | FAN Yi-sun ZENG Wan-jie GENG Chun-song HU Zhen-hua CHEN Qiang DING Shuang-shuang |
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| Affiliation: | FAN Yi-sun,ZENG Wan-jie,GENG Chun-song,HU Zhen-hua,CHEN Qiang,DING Shuang-shuang(Department of Clinical Laboratory,Suzhou Kowloon Hospital,Shanghai Jiao Tong University Medical School,Suzhou 215021,Jiangsu,China) |
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| Abstract: | Objective To develop a multiplex flow method for the detection of antibodies against MICB(major histocompatibility complex class I chain related molecule B),and analyze the distribution frequency of MICB antibodies in healthy adults.Methods Recombinant proteins of MICB were produced by insect cell expression system.The purified MICB molecules were coupled to Luminex beads.The linear range,specificity,precision and accuracy of the detection system were evaluated.A total of 500 samples of healthy individuals ... |
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| Keywords: | MICB Luminex polymorphism antibody |
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