Alzheimer病及多梗塞性痴呆患者血清载脂蛋白A I、B100、E水平及apoE多态性分析

目的 探讨中国人血清载脂蛋白（apo)AI、B100、E水平及apoE多态性与Alzheimer病（AD）及多梗塞性痴呆（MID）的关系。方法 采用单向免疫扩散法及等电聚焦免疫印迹分析法对75例AD、36例MID和60例对照受检者的血清apoA I、B100、E及apoE多态性进行了分析。结果 AD组及MID组apoE表型E4（E4／4＋E3／4＋E2／4）的频率分别为0．3466和0．3889，较对照组的0．1167明显为高（P＜0．05），ε4等位基因频率分别为0．2333和0．2083，均明显高于对照组（0．0666）（P＜0．05），AD组ε2等位基因频率低于对照组（0．0467vs 0．0833，P＜0．05）；AD组及MID组患者血清apoA I及E水平明显低于对照组（P＜0．001，P＜0．05）。结论 apoEε4等位基因与中国人AD和MID有关联，AD及MID患者血清apoA I及E水平明显降低（P＜0．001及P＜0．05）。

Serum apolipoprotein A I, B100 and E levels and apolipoprotein E polymorphism in patients with Alzheimer's disease and multiple infarction dementia in Chinese population]

OBJECTIVE: To investigate the relationship of serum apolipoprotein (apo) A I, B100 and E levels and apo E polymorphism to Alzheimer's disease (AD) and multiple infarction dementia (MID) in Chinese population. METHODS: The apoE phenotypes were assayed by isoelectric focusing and immunoblotting, and apolipoproteins were determined by radial immunodiffusion assay in 75 patients with AD, 36 patients with MID and 60 control subjects. RESULTS: The frequency of apo E4 allele (epsilon 4) was significantly higher and the frequency of epsilon 2 allele was lower in AD group, compared with those in the control group (0.2333 vs 0.0666, 0.0467 vs 0.0833, P < 0.05). The frequency of epsilon 4 allele in MID group was also higher than that in the control group (0.2083 vs 0.0666, P < 0.05). The fasting serum apoA 1 and E levels in AD and MID groups were remarkably lower than those in the control group (P < 0.001 and P < 0.05). CONCLUSION: Apo E4 allele epsilon 4 was associated with AD and MID. ApoE4 might be a risk factor for AD and MID, and apo E2 might be a protective factor for AD. The serum apo A I and apoE levels were significantly decreased in patients with Alzheimer's disease and multiple infarction dementia.