| ENK与CaBPs在PPE-GFP转基因小鼠视网膜细胞中的共存 |
| |
| 引用本文： | 韩蕊,黄静,陈晶,汪伟,李云庆,武胜昔.ENK与CaBPs在PPE-GFP转基因小鼠视网膜细胞中的共存[J].神经解剖学杂志,2010,26(1). |
| |
| 作者姓名： | 韩蕊 黄静 陈晶 汪伟 李云庆 武胜昔 |
| |
| 作者单位： | 第四军医大学基础部:人体解剖与组织胚胎学教研室暨梁鲸锯脑研究中心,西安,710032 |
| |
| 摘 要： | 目的:以PPE-GFP转基因小鼠为研究工具,观察绿色荧光蛋白(GFP)阳性的脑啡肽(ENK)能神经元与钙结合蛋白D28K(CB)、钙视网膜蛋白(CR)和小白蛋白(PV)等钙结合蛋白(CaBPs)成员在视网膜的分布及共存情况。方法:利用免疫组织化学和免疫荧光双标染色的方法。结果:GFP阳性的ENK能细胞主要分布在视网膜内核层内缘,少量分布在节细胞层。所有的GFP阳性细胞均与神经元标志物NSE共存,但不与星形胶质细胞标志物GFAP共存。GFP与CB、CR和PV均有部分共存,其中GFP/CB共存神经元占GFP阳性细胞的8.65%,占CB阳性细胞的5.84%;GFP/CR共存神经元占GFP阳性细胞的18.18%,占CR阳性细胞的14.28%,且共存细胞仅见于内核层;GFP/PV共存细胞占GFP阳性细胞的68.75%,占PV阳性细胞的91.67%,共存细胞主要位于内核层,少量见于节细胞层。结论:ENK能神经元在视网膜内具有板层特异性的分布特点和与钙结合蛋白成员有不同的共存模式,上述结果为深入研究小鼠视网膜ENK能神经元的功能意义提供了形态学依据。
|
| 关 键 词： | 脑啡肽 前脑啡肽原 绿色荧光蛋白 钙结合蛋白 视网膜 转基因小鼠 |
Coexistence of enkephalin with calcium-binding proteins in the retinal cells of the PPE-GFP transgenic mouse |
| |
| Han Rui,Huang Jing,Chen Jing,Wang Wei,Li Yunqing,Wu Shengxi.Coexistence of enkephalin with calcium-binding proteins in the retinal cells of the PPE-GFP transgenic mouse[J].Chinese Journal of Neuroanatomy,2010,26(1). |
| |
| Authors: | Han Rui Huang Jing Chen Jing Wang Wei Li Yunqing Wu Shengxi |
| |
| Institution: | Han Rui,Huang Jing,Chen Jing,Wang Wei,Li Yunqing,Wu Shengxi(Department of Human Anatomy,Histology , Embryology,K.K.Leung Brain Research Centre,Fourth Military Medical University,Xi\'an 710032,China) |
| |
| Abstract: | Objective:The present study was designed to observe the distribution and coexistence of enkephalin(ENK)and part of calcium-binding proteins including calbindin D28K(CB),calretinin(CR)and parvalbumin(PV)in the retina of the PPE-GFP transgenic mouse.Methods:Immunohistochemistry and double immunofluorescence labeling methods were used.Results:GFP-positive cells were mainly distributed in the inner part of the inner nuclear layer(INL)and some of them were also located in the ganglion cell layer(GCL).None of the... |
| |
| Keywords: | enkephalin preproenkephalin green fluorescent protein calcium bindin protein retina transgenic mouse |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
