P27kipl、CyclinD1在膀胱癌中的表达及其意义

目的:通过检测P27kipl与CyclinD1在膀胱癌中的表达,以探讨两者在膀胱癌发生发展中的作用及其与临床病理特征的关系.方法:对80例标本进行SP免疫组织化学方法检测P27kipl、CyclinD1的表达水平,其中正常组织28例,膀胱癌组织52例.结果:P27kipl、CyclinD1在正常膀胱组织中的表达率和在膀胱癌组织中的表达差异均具有显著性(P＜0.01).P27kipl蛋白在低分化组中的表达水平显著低于高分化组(P＜0.01)和中分化组(P＜0.01),CyclinD1表达水平在低分化组中显著高于高分化组(P＜0.01),在中、低分化组间差异无显著性(P＞0.05).P27kipl的表达与临床分期无关,而CyclinD1的表达与临床分期相关.P27kipl与CyclinD1的相互关系呈负相关.结论:P27kipl蛋白的低表达或缺失在膀胱癌的发生中起重要作用,是膀胱癌发生中的早期分子事件.CyclinD1的过表达参与了膀胱癌的发生,并与其发展密切相关,可作为评价预后的指标之一.膀胱癌的发生发展与P27kipl、CyclinD1蛋白的异常表达密切相关,联合测定P27kipl、CyclinD1蛋白的表达对膀胱癌的诊断、治疗有一定的指导意义,可作为膀胱癌诊断和预后评价的重要参数.

Expressions of P27kipl and CyclinD1 in urinary bladder cancer

Objective To explore the roles of P27kipl and CyclinD1 in the oncogenesis of urinary bladder cancer and the relationship between these two genes and the pathological characteristics of bladder cancer by detecting the expressions of P27kipl and CyclinD1. Methods The levels of P27kipl and CyclinD1 expression in 80 specimens (28 normal tissue, 52 bladder cancer tissue) were detected by SP immunohistochemistry. Results There was a significant difference in the P27kipl and CyclinD1 between the normal bladder tissue and the bladder cancer tissue (P< 0.01). The level of P27kipl expression in the poorly differentiated tissue was markedly lowered than that in the moderately or well- differentiated tissue (P< 0.01). The level of CyclinD1 expression in the poorly differentiated tissue was much higher than that in the well- differentiated tissue (P< 0.01) but there was little difference in the CyclinD1 between the poorly differentiated and medium- differentiated tissue (P >0.05). The CyclinD1 expression was associated with the clinical stage whereas the P27kipl expression was not. P27kipl was negatively related to CyclinD1. Conclusion Low P27kipl expression or loss of expression plays an important role in the oncogenesis of urinary bladder cancer and is an early molecular pattern in the procession. Overexpression of CyclinD1 is also involved in the occurrence of bladder cancer, associated with its progression, and can be an indication for the assessment of prognosis. The oncogenesis and progression of bladder cancer are closely related to the abnormal expressions of P27kipl and CyclinD1. Detection of P27kipl plus CyclinD1 expression is of some significance in the diagnosis and treatment of urinary bladder cancer and may be an important parameter in the diagnosis and prognosis assessment of the malignancy.