| HLA基因DRB1*150106的发现及家系调查分析 |
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| 引用本文： | 张伯伟,赵磊,杨瑞云,杜娟,马茹.HLA基因DRB1*150106的发现及家系调查分析[J].河南医学研究,2006,15(4):302-304. |
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| 作者姓名： | 张伯伟 赵磊 杨瑞云 杜娟 马茹 |
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| 作者单位： | 河南省红十字血液中心,河南郑州,450012 |
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| 基金项目： | 新基因的测序、申报工作在美国国家骨髓库海军实验室完成.特此感谢!. |
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| 摘 要： | 目的:用DNA测序的方法确定新的HLA等位基因,调查分析该基因的遗传情况。方法:用SSO流式荧光微技术HLA分型技术进行HLA-A、B、DRB1中低分辨查测对疑为新基因的样本用DNA测序方法确定新的基因,并对该新基因携带者家系调查。结果:用流式荧光微珠法HLA分型检测出现无法解释的结果,怀疑为新的基因序列,经对怀疑为新基因第二外显子进行测序分析,确定为新的基因序列,与之相近序列等位基因DRB1*150101相比,在2外显子164 A>T,家系分析该新基因来自受检者母亲一方。结论:该新基因已由WHO HLA因子命名委员会注册,命名为DRB1*150106(DQ514601、HW10003803),该新基因的产生机制及生物等功能有待进一步研究。
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| 关 键 词： | HLA 新基因 家系调查 |
| 文章编号： | 1004-437X（2006）04-0302-03 |
| 收稿时间： | 2006-11-15 |
| 修稿时间： | 2006-12-01 |
Discovery of a new allele HLA-DRB1 * 150106 and investigate of its family heritage |
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| ZHANG Bo-wei,ZHAO Lei,YANG Rui-yun,DU juan,MA Ru.Discovery of a new allele HLA-DRB1 * 150106 and investigate of its family heritage[J].Henan Medical Research,2006,15(4):302-304. |
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| Authors: | ZHANG Bo-wei ZHAO Lei YANG Rui-yun DU juan MA Ru |
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| Institution: | Henan Red Cross Blood Center, Zhengzhou 450012, China |
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| Abstract: | Objective:Identify a new HLA DRB1 allele by sequencing and investigate the heritage of this new allele.Methods: An DRB1 ambiguous result was found in the routine HLA-A、B、DRB1 typing using SSO flexible muti-analyte profiling(LAB TYPE SSO).This new was conformed by DRB1 axon 2 sequencing,family investigation was preformed.Results: An A>T was found at 164 axon 2 of DRB1 compare with DRB1*150101 which was found earlier.Family investigation discover,the new allele comes from donor's mother.Conclusion: This new sequence was first found and has been officially assigned HLA-DRB1150106 by the WHO nomenclatures committee. |
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| Keywords: | HLA new allele family heratige investigion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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