| 重症监护病房感染阴沟肠杆菌的耐药性及基因型分析 |
| |
| 引用本文： | 戴春梅,陈辉,袁金玲.重症监护病房感染阴沟肠杆菌的耐药性及基因型分析[J].江西医学院学报,2006,46(3):20-22. |
| |
| 作者姓名： | 戴春梅 陈辉 袁金玲 |
| |
| 作者单位： | 中南大学湘雅三医院检验科,湖南长沙410013 |
| |
| 摘 要： | 目的了解我院重症监护病房（ICU）医院感染阴沟肠杆菌的耐药谱及分子流行病学特征，指导临床合理用药。方法基因分型采用优化反应体系的随机扩增多态性DNA（RAPD）法，耐药分析选用K—B法。结果RAPD分型将28株阴沟肠杆菌分为11种株型，耐药谱则将其分为12种不同的耐药型。结论基因分型和耐药分析显示ICU有多重耐药的阴沟肠杆菌株爆发流行现象，它为控制阴沟肠杆菌的医院内感染、追踪传染原、切断传播途径提供遗传学信息．指导临床医生选用敏感有效的抗生素。
|
| 关 键 词： | 阴沟肠杆菌 医院感染 基因分型 耐药性 |
| 文章编号： | 1000-2294(2006)03-0020-03 |
| 收稿时间： | 02 21 2006 12:00AM |
| 修稿时间： | 2006年2月21日 |
Drug Resistance and Genotyping of Enterobacter Cloacae Infected in Intensive Care Units |
| |
| DAI Chun-mei,CHEN Hui,YUAN Jin-ling.Drug Resistance and Genotyping of Enterobacter Cloacae Infected in Intensive Care Units[J].Acta Academiae Medicinae Jiangxi,2006,46(3):20-22. |
| |
| Authors: | DAI Chun-mei CHEN Hui YUAN Jin-ling |
| |
| Institution: | The Clinical Laboratory of the Third Xiangya Hospital of Central South University, Changsha , 410013, China |
| |
| Abstract: | Objective To study the type of resistance spectrum and molecular biological characteristics of Enterobacter cloacae infected in intensive care units.Methods Random amplified polymorphic DNA(RAPD)analysis was used for gene typing.K-B method was used for the determination of antibiotic resistance pattern.Results Twenty-eight clinical strains of E.cloacae were divided by RAPD into 10 types.And according to the antibiotic resistance patterns,the 28 strains were typed into 12 kinds.Conclusion Genotyping and analysis of resistance to antibiotics demonstrate the outbreak of multidrug resistant strains E.cloacae in intensive care units.It can not only provide genetic information for controlling,preventing hospital infection and tracing the infection source and the way of dissemination but also direct the clinical doctors to select sensitive antibiotics. |
| |
| Keywords: | E cloacae hosptal infection genotype resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
