Inhibition of medium and short-chain fatty acid oxidation in rat heart mitochondria by dichloroacetate |
| |
Authors: | Kwok-Chu Man John T. Brosnan |
| |
Affiliation: | Department of Biochemistry, Memorial University of Newfoundland, St. John''s, Newfoundland, A1B 3X9 Canada. |
| |
Abstract: | The effect of dichloroacetate (DCA) on the oxidation of medium-chain and short-chain fatty acids was studied in rat heart mitochondria. Dichloroacetate (1–10 mM) markedly decreased medium-chain and short-chain fatty acid oxidation (oxygen uptake and release of 14CO2 from labelled fatty acids) by heart mitochondria; the oxidation of long-chain fatty acid, however, was unaffected by the compound. The oxidation of acylcarnitines was not influenced by DCA. Dichloroacetate inhibited medium-chain and short-chain fatty acid oxidation by inhibiting the activity of mitochondrial acyl-CoA synthases. Inhibition of the enzyme activity by DCA was rapid and not due to its effect on pyruvate dehydrogenase. |
| |
Keywords: | Address reprint request to Dr. John T. Brosnan Department of Biochemistry Memorial University of Newfoundland St. John's Newfoundland Canada A1B 3X9. |
本文献已被 ScienceDirect 等数据库收录! |