微板法测定淡色库蚊体内非特异性酯酶检测其对杀虫剂抗性的研究

目的　探讨简捷灵敏的蚊虫抗药性早期测报技术。方法　以 β -乙酸萘酯为底物 ,坚固蓝B盐为显色剂 ,用微板法测定实验室和现场单个淡色库蚊体内非特异性酯酶活力。结果　实验室五个品系淡色库蚊以抗DDVP品系非特异性酯酶活力水平最高 ,其次为抗残杀威品系和抗DDVP降解品系 ,抗氯氰菊酯品系较低 ,与敏感品系相近 ;三个现场淡色库蚊种群对DDVP抗性为 4 15～ 9 36倍 ,对残杀威抗性为 1 0 2～ 3 81倍 ,其非特异性酯酶活力水平均较高。结论　微板法测定非特异性酯酶能够检测蚊虫对有机磷和氨基甲酸酯类杀虫剂的抗性水平。方法简便快速 ,结果客观准确 ,可重复性强 ;可测出抗性频率 ,早期发现抗性的存在 ;可测知抗性机制 ,便于制订抗药性克服对策

Microplate test of non-specific esterase of Cx.pipiens pallens for insecticide resistance detection

Aim To explore simple and sensitive technic for insecticide resistance detection and early prediction Methods Microplate test was used to detect non specific esterase(NSE)of single Cx pipeins pallens of laboratory and field population,β Naphthyl acetate was used as the substrate and fast blue B salt solution as the colour agent Results The NSE level was highest in anti DDVP strain,followed by anti propoxur strain and anti DDVP degenerated strain,while the level was similar between anti cypermethrin and susceptible strain Three field collected Cx pipiens pallens populations have 4 15-9 36 fold higher resistance against DDVP and 1 02-3 81 fold higher resistance against propoxur than susceptible mosquito,and their NSE level was also higher than susceptible strain Conclusion Microplate test for NSE can be used for the discrimination of resistance against Op and carbamate insecticides The method is easy and rapid to operate with high precision and objective results Resistance frequency and mechanism can be detected at the same time,so early prediction of resistance and working out resistance management measure is possible