口腔粘膜鳞癌发生发展过程中P53基因,P53蛋白及PCNA抗原…

两两多元相关分析发现，ＰＣＲ－ＳＳＣＰ分析异常、Ｐ５３染色阳性与ＰＣＮＡ指数之间无论是在口腔粘膜癌前损害、口腔粘膜癌原发灶或淋巴结转移灶中，均有明显的相关关系；将患者的性别、年龄共同引入进行多元判别分析，获得利用指标进行口腔粘膜癌前损害量的分度、膜鳞癌病理分级、以及判定口腔粘膜鳞癌患者是否淋吉转移的多元判别方程初步的判别结果显示其判别准确率分别达８２．５％，７８％，８０％，说明这些方程已具备了临床

A study on the significance of p53 gene mutation, P53 protein positive staining and PCNA staining]

After obtaining the results of p53 gene mutation by a silver staining method to polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, P53 protein staining and proliferation cell nuclear antigen (PCNA) index by double-blind method, multivariate correlation analysis two by two showed that PCR-SSCP, P53 positive protein staining and PCNA index had markedly correlation not only in oral precancerous lesions (OPL) but also in primary sites and regional metastatic lymph nodes of oral squamous cell carcinomas (OSCC). This indicated that the nature of the three indexes was the same. After introducing the sex and age of patients which have been proved to be related to the initiation and development of OPL and OSCC according to other researchers and are easy to be obtained in clinics, multivariate discriminatory analysis was carried on and three groups of discriminatory equations were gotten about the pathological grading of OPL and OSCC and metastasis condition of OSCC. The accuracy of these equations was 82.5%, 78% and 80%, individually. This suggests that the equations have applicable potentiality already.