连梅饮中黄芩苷和小檗碱的含量测定

目的探讨连梅饮中黄芩苷和小檗碱的含量测定方法。方法采用RP—HPLC／UV法测定连梅饮中黄芩苷和小檗碱的含量；色谱柱：Agilent HC C18柱（250×4．6mm，5μm）；乙腈：0．05mol／L磷酸二氢钾溶液梯度洗脱；检测波长275nm；流速1ml／min。结果黄芩苷在0．448—112μg／ml的浓度范围，小檗碱在0．424～106μg／ml的浓度范围内线性良好：低和高浓度的黄芩苷和小檗碱的精密度（RSD）分别为3．88％、4．84％、4．63％、2．29％；加样回收率分别为（104．3±1.3）％、（97．7±6．9）％；连梅饮中黄芩苷的含量为（2．67±0．012）mg／ml，RSD为0．45％（n=3）；小檗碱的含量为（2．47±0．014）mg／ml，RSD为2．98％（n=3）。结论本方法快速、简便、准确，可用于测定制剂中黄芩苷和小檗碱的含量。

Determination of Baicalin and Berberine in Lianmeiyin preparation

Objective The determination method of Baicalin and Berberine in Lianmeiyin preparation was established. Methods The RP-HPLC/UV method was determined, as follows： Agilent HC C18 column （250×4.6mm,5μm）, acetonitrile and 0.05mol/L KH2PO4 solution linearity gradient elution, flow rate at 1ml/min, UV detection at 275nm. Results An excellent linearity of Baicalin and Berberine was obtained over the range of 0.448±112 and 0.424±106μg/ml, respectively. The RSDs of Baicalin and Berberine at different concentration were 3.88%,4.84%,4.63% and 2.29%, respectively. The mean recovery rates of Baicalin and Berberine in preparation were （104.3±1.3）% and （97.7±6.9）% ,respectively. The Baicalin and Berberine in Lianmeiyin preparation were （2.67±0.012） mg/ml, with RSD 0.45%（n=3） and （2.47±0.014）mg/ml, with RSD 2.98%（n=3）. Conclusion The method is simply, reliable and rapidness. It＇ s quite suitable to be used in the analysis of Baicalin and Berberine in Lianmeiyin preparation.