| 羊水细胞ABO血型PCR-SSCP法基因定型研究 |
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| 引用本文： | 林春燕,袁晖,王晨虹,谢建生,林胜谋,郝颖,何卫恒,李辉.羊水细胞ABO血型PCR-SSCP法基因定型研究[J].中国热带医学,2008,8(7):1099-1100. |
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| 作者姓名： | 林春燕 袁晖 王晨虹 谢建生 林胜谋 郝颖 何卫恒 李辉 |
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| 作者单位： | 深圳市妇幼保健院,广东,深圳518028 |
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| 摘 要： | 目的分析以羊水细胞进行胎儿ABO血型基因分型及其可行性分析。方法妊娠16周孕妇抽取羊水,从羊水细胞中提取DNA,采用PCR-SSCP技术,通过PCR反应,特异性扩增ABO基因片段,用凝胶电泳检测PCR产物,SSCP硝酸银染色,PCR产物直接测序等分子生物学方法,直接鉴定ABO血型基因型。结果15例高抗体效价的O型孕妇中丈夫A型的9例,B型5例,AB型1例,胎儿羊水细胞基因定型血型与末梢血血型吻合。结论通过羊水细胞,采用PCR-SSCP技术,进行胎儿ABO血型基因定型的方法是可行的。
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| 关 键 词： | 羊水细胞 ABO血型 基因定型 |
PCR-SSCP genotyping of ABO blood group of amniocyte |
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| Institution: | LIN Chun-yan;YUAN Hui;WANG Chen-hong;et al.(Shenzhen Municipal Maternal Hospital;Shenzhen 518028;Guangdong;P.R.China) |
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| Abstract: | Objective To invbestigate the feasibility of genotyping of ABO blood group in amniocyte.Methods After distilled DNA from women with a gestational period of sixteen weeks and with blood type O.The ABO blood group were identified directly by PCR-SSCP.Results Among fifteen husbands of those pregnant women,9 were with blood type A,five with blood type B,one with blood type AB.The genotyping of ABO blood type detected by PCR-SSCP were coincidence with that detected by using capillary blood.Conclusion It is feasible to detect genotyping of fetal ABO blood group in amniocyte by PCR-SSCP. |
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| Keywords: | Amniocyte ABO blood group Genotype |
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