脑尔康对Alzheimer病小鼠脑细胞凋亡相关基因表达的影响

目的　观察复方中药脑尔康对 Alzheimer病 (AD)模型小鼠 ,学习记忆障碍的改善作用及对凋亡相关基因的调控作用。方法　用跳台法测试各组小鼠学习记忆功能 ,用 SP免疫组化法检测 Fas、Bcl- 2阳性细胞计数。结果　模型组与空白对照组比较 ,触电时间延长 ,潜伏期缩短 ,错误次数增多 ;Fas、Bcl- 2表达升高 (均 P<0 .0 1 )。各用药组与模型组比较触电时间缩短 ,潜伏期延长 (均 P<0 .0 5) ,Fas、Bcl- 2表达下调 (P<0 .0 5或 P<0 .0 1 )。各参数中药中、小剂量组优于西药组。结论　脑尔康能改善 AD小鼠学习记忆障碍具有促智作用 ,可通过调控凋亡相关基因表达 ,抗脑细胞凋亡 ,减少神经元丢失 ,而发挥抗痴呆作用

The effects of Nao Er Kang on expressions of apoptosis-related genes in AD mice brain

Objective To evaluate effects of Nao Er Kang on learning and memory disorders in Alzheimer's disease (AD) mice caused by AlCl 3 and the regulation of expressions of apoptosis-related genes in brain cells.Methods The learning and memory functions were detected in every group, and the numbers of Fas, Bcl-2 positive cells were tested by SP immunohistochemistory.Results In model group, the touch electricity time prolonged, the latent period shortened and the error times increased compared with control group. Compared with model group, the touch electricity time shortened, the latent period prolonged and the error times decreased in three Nao Er Kang groups ( P <0.05, both), and the expression of Fas and Bcl-2 in mice brain cells reduced ( P <0.05 or P <0.01). The groups of Nao Er Kang with middle and little dose were prior to the group treated with western medicine in all parameters of above. Conclusions Nao Er Kang could protect mice from learning and memory disorders caused by AlCl3 by regulating expression of apoptosis-related genes in AD mice brain so that it had anti-dementia effects.