抗体鸡尾酒AMACR/P63/34βE12在前列腺良恶性病变鉴别诊断中的应用价值

目的:探讨抗体鸡尾酒AMACR/P63/34βE12在前列腺良恶性病变鉴别诊断中的应用价值。方法:收集2001~2005年111例前列腺手术切除标本，其中前列腺腺癌39例，高级别前列腺上皮内瘤（high-grade prostatic intraepithelial neoplasias，HGPIN）29例，非典型性腺瘤样增生（atypical adenomatous hyperplasia, AAH）3例，前列腺结节性增生（benign prostatic hyperplasia, BPH）40例。作抗体鸡尾酒AMACR/P63/34βE12的免疫标记，观察3种抗体在各类病变中的表达情况。结果:39例前列腺腺癌AMACR全部呈阳性，癌巢周围无基底细胞残存（P63/34βE12阴性）。29例高级别前列腺上皮内瘤变，14例（48.3%）腺泡上皮AMACR呈阳性，29例腺泡上皮周围有连续或不连续的基底细胞（P63/34βE12阳性）。3例非典型性腺瘤样增生中2例腺泡上皮AMACR呈弱阳性；3例腺泡上皮周围有较连续的基底细胞（P63/34βE12中度阳性）。40例前列腺结节性增生，腺泡上皮AMACR染色均呈阴性，周围有连续的基底细胞（P63/34βE12强阳性）。结论:鸡尾酒抗体AMACR/P63/34βE12标记前列腺组织，能够同时高特异性和敏感性地检测出前列腺腺癌细胞（或非典型增生的腺泡上皮细胞）和基底细胞，为前列腺腺癌与高级别上皮內瘤变、非典型性腺瘤样增生、前列腺结节性增生的鉴别诊断提供有力的证据。

The value of using an AMACR/P63/34βE12 Cocktail staining in differential diagnosis of prostate benign and malignant diseases

ObjectiveTo investigate the value of using an AMACR/P63/34βE12 cocktail antibody staining in differential diagnosis of benign and malignant diseases of prostate. MethodsCollect 111 prostatectomy specimens cases during 2001~2005 , including 39 prostate adenocarcinoma specimens,29 high-grade prostatic intraepithelial neoplasias (HGPIN) specimens, 3 atypical adenomatous hyperplasia (AAH) specimens, 40 benign prostatic hyperplasia (BPH) specimens.3-cocktail antibody (AMACR/P63/34βE12) were used to detect different prostate lesions. ResultsOf 39 prostate adenocarcinoma cases ,all cases expressed AMACR, all malignant glands were negative for basal cell staining. Fourteen of 29 HGPIN cases,2 of 3 AAH cases espressed AMACR, but there were basal cells around the glandular epithelium of all HGPIN,AAH,BPH cases. There were no any BPH cases expressing AMACR. ConclusionAMACR/P63/34βE12 cocktail antibody could detect prostatic glandular epithelium cells and basal cells with high sensibility and specificity , there were important value for the diagnosis of prostate adenocarcinoma.