榄香烯体外诱导急性白血病细胞凋亡的研究

目的 探讨榄香烯诱导白血病细胞凋亡的作用及其机制。方法 采用MTT法、荧光显微镜、透射电镜、流式细胞术（FCM）、DNA电泳方法观察榄香烯对HL．60白血病细胞株的促凋亡作用，并进行细胞周期分析和采用RT-PCR、FCM检测bcl-2基因表达的变化。结果 榄香烯在体外诱导HL-60细胞凋亡，呈细胞凋亡典型的形态和生化特征，可见凋亡小体和梯形条带，并具有时间剂量依赖性，凋亡率最高达51．8％，在凋亡过程中细胞阻滞于S期，并检测到bcl-2基因表达下调。结论 榄香烯具有诱导细胞凋亡的作用，其机制可能与bcl-2基因表达下调有关。

Experimental Study of Elemene Induced Leukemia Cells Apoptosis

Objective To elucidate the apoptosis of leukemia cells induced by elemene and its mechanism. Methods The inhibition of leukemia cells line HL-60 was tested by MTT assay, and morphological assessment and the HL-60 cells undergoing apoptosis was determined by fluorescent microscope and electron microscope. The endonucleolysis of HL-60 was analyzed by agarose gel electrophoresis and flow cytometry. The expression of bcl-2 gene was determined by FCM and RT-PCR. Results The apoptosis of HL-60 cells was induced by elemene in vitro with a manner of time and dosage dependence. Apoptosis body and DNA ladder pattern were observed, and apoptosis peak reached 51.8%. While apoptosis, cells were arrested at G1/S check point, downregulation of bcl-2 expression was evaluated by RT-PCR and FCM. Conclusion Elemene induced apoptosis of HL-60 cells, associated with downregulation of bcl-2 gene expression.