db/db自发性糖尿病小鼠睾丸生殖细胞增殖及凋亡研究

目的　研究糖尿病对生殖细胞增殖和凋亡的影响。　方法　以免疫组织化学ABC法检测增殖细胞核抗原 (proliferationcellnucleusantigen ,PCNA)检测生殖细胞的增殖情况 ,电镜和脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记法 (TUNEL)检测凋亡的生殖细胞。　结果　PCNA阳性率随糖尿病病程进展 (糖尿病组 )和年龄增加 (对照组 )均呈明显下降趋势 ,但每年龄段糖尿病组PCNA阳性率均明显低于相应正常对照组。凋亡管横断面数及凋亡阳性率糖尿病组均较正常高 ,而且随糖尿病病程进展呈明显增加趋势 ,对照组中随加龄二者有增加。电镜下 ,糖尿病组小鼠睾丸精原细胞、精母细胞和精子细胞均可见到核染色质凝集、边集 ,凋亡小体形成和降解等凋亡过程。　结论　db/db自发性糖尿病小鼠睾丸生殖细胞PCNA表达减弱 ,TUNEL标记凋亡细胞增加 ,说明糖尿病使生殖细胞增殖与凋亡的平衡破坏 ,这可能是糖尿病生殖功能障碍的原因

THE STUDY OF PROLIFERATION AND APOPTOSIS IN db/db MUTANT DIABETIC MICE TESTIS

Objective To investigate the effect of diabetes mellitus on the germ cell proliferation and apoptosis in C57b1/Ksj db/db mutant diabetic mice testis. Methods The proliferation was marked with proliferation cell nucleus antigen(PCNA) by immunohistochemistry. Apoptosis was labeled with TUNEL and electron microscope observation. Results The positive rate of PCNA significantly decreased with the diseases progress in DM groups. In controls, the same reduced feature was found. However, the positive rate of DM group was significant lower than those of controls. The positive tubules and apoptotic rate in DM groups were significant higher than that of age matched controls. Apoptotic spermatogonium, spermatocyte or spermatozoon contained marginated, condensed chromatin and an apoptotic body.Conclusion In db/db diabetic mice, the reduced expression of PCNA and increasing TUNEL positive apoptotic germ cells demonstrated that DM induced apoptosis and inhibited proliferation, which might be one reason of the infertility during diabetes mellitus.