反义寡核苷酸封闭癌基因MDM2提高白血病细胞化疗敏感性的研究

目的通过MDM2反义寡核苷酸（MDM2-AS）阻滞白血病K562细胞MDM2的表达，研究非p53通路下MDM2抑制的化疗增敏作用。方法细胞分为对照组、AS组、顺铂组和联合组，以RT—PCR和Western blot检测MDM表达，流式细胞仪检测处理后各组细胞的凋亡率。结果AS组细胞MDM2表达明显受抑制（P〈0．05），联合组K562细胞凋亡率明显增加（P〈0．01）。结论在p53缺失情况下，以MDM2-AS反义封闭抑制MDM2表达，可增加白血病细胞的化疗敏感性，为白血病的临床治疗提供参考。

Study of chemotherapeutic sensitization of leukemia cells with MDM2-AS transfected into leukemia cells of K562

Objective] Chemotherapeutic sensitization of MDM2 antisense Oligonuleotides(MDM2-AS) is studied with MDM2-AS transfected into leukemia cells of K562.Methods] All the cells were divided into control group,MDM2-AS group,Cisplatin group,and integrated treatment group.Western blot and RT-PCR were used to detect MDM2 expression,and flow cytometer was used to detect apoptosis rates of all groups cells.Result] MDM2 expression was inhibited obviously after MDM2-AS transfection(P<0.05).Treated with MDM2-AS and Cisplatin together,K562 cells apoptosis rate increased obviously(P<0.01).Conclusion] MDM2 inhibition with MDM2-AS transfection in no-p53 expression cells can improve chemotherapeutic sensitization of leukemia cells,it indicates wide use of this treatment in leukemia chemotherapy.