| HPLC法测定万寿菊茎中叶黄素的含量 |
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| 引用本文： | 曹洪昭,聂传平,李红玉. HPLC法测定万寿菊茎中叶黄素的含量[J]. 数理医药学杂志, 2010, 23(2): 197-199. DOI: 10.3969/j.issn.1004-4337.2010.02.031 |
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| 作者姓名： | 曹洪昭 聂传平 李红玉 |
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| 作者单位： | 辽宁医学院药学院,锦州,121001 |
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| 摘 要： | 目的:建立一种测定万寿菊茎中叶黄素含量的HPLC方法。方法:采用Diamonsil-C18色谱柱(200mm×4.6mm,5μm),流动相:甲醇∶水=97.5∶2.5,流速:1.0mL/min,检测波长:445nm。结果:叶黄素在1.92～96μg/mL范围内,有良好的线性关系(r=0.9996),平均回收率为98.9%,RSD1.04%。结论:该法简便准确、重现性好,可用于万寿菊茎中叶黄素的含量测定。
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| 关 键 词： | 万寿菊茎 叶黄素 高效液相色谱 含量测定 |
Determination of Lutein in Stem of Marigold by HPLC |
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| Cao Hongzhao,et al. Determination of Lutein in Stem of Marigold by HPLC[J]. Journal of Mathematical Medicine, 2010, 23(2): 197-199. DOI: 10.3969/j.issn.1004-4337.2010.02.031 |
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| Authors: | Cao Hongzhao et al |
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| Affiliation: | Cao Hongzhao,et al(College of Pharmacy,Liaoning Medical University,Jinzhou 121001) |
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| Abstract: | Objective： To establish a HPLC method for the simultaneous determination of lutein in Stem of Marigold. Methods. The chromatographic column was Diamonsil-C18 （200 mm×4. 6 mm, 5 μm）, mobile phase. MeOH ： H2O= 97. 5 ： 2. 5, flow rate： 1.0 mL/min and detection wave-length： 450 nm. Results： The linearity of lutein was obtained separately over the range of 1.92--96 μg/mL, r=0. 999 6. The average recovery rates of lutein was 98. 9% with corresponding RSD of 1.04%. Conclusion： The HPLC method with good reproducibility was simple and reliable for the simultaneous determination of lutein in Stem of Mari-gold. |
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| Keywords: | marigold dried stem lutein HPLC determination |
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