Infectivity studies of bromegrass mosaic virus RNA |
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| Authors: | L E Bockstahler P Kaesberg |
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| Affiliation: | 1. Department of Environmental Health, School of Public Health, China Medical University, Shenyang 110122, China;2. Department of Immunology and Rheumatology, First Hospital, China Medical University, Shenyang 110001, China;3. Department of Anal and Rectal Diseases, First Hospital, China Medical University, Shenyang 110001, China;4. Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China;1. University of Coimbra, Centre for Functional Ecology - Science for People & the Planet, Department of Life Sciences, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal;2. University of Coimbra, Centre for Social Studies, Colégio São Jerónimo, Apartado 3087, 3000-995 Coimbra, Portugal;1. Division of Civil Engineering, Faculty of Engineering, Bangkokthonburi University, 16/10 Leabklongtaweewatana Road, Taweewatana, Bangkok 10170, Thailand;2. Department of Building Technology, Faculty of Industrial Technology, Phranakhon Rajabhat University, 9 Changwattana Road, Bangkhen, Bangkok 10220, Thailand;1. Department of Materials Science and Engineering, China University of Petroleum-Beijing, Beijing 102249, China;2. Beijing Key Laboratory of Failure, Corrosion, and Protection of Oil/Gas Facilities, China University of Petroleum-Beijing, Beijing 102249, China;1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA;2. Department of Integrative Biology, Oregon State University, Corvallis, OR 97331, USA |
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| Abstract: | The infectivity of bromegrass mosaic virus (BMV) RNA has been investigated. The infectivity per unit weight of unfractionated RNA was approximately one-third that of an equivalent weight of RNA in the virus. No significant loss of infectivity occurred during 70 hours of incubation at 30°. BMV RNA is known to consist of three components of molecular weights 0.3 × 106, 0.7 × 106, and 1.0 × 106. These components were separated by sucrose density-gradient centrifugation. It was found that infectivity was associated only with the 1.0 × 106 molecular weight component; the two smaller components were noninfectious when tested either separately or in combination. An enhancement of infectivity was observed when the infectious component was inoculated in the presence of either of the smaller components or in the presence of unrelated RNAs. |
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