白藜芦醇对血管平滑肌细胞增殖的影响

目的　观察白藜芦醇 (resveratrol,Res)对体外培养的大鼠主动脉血管平滑肌细胞 (VSMC)的增殖、DNA合成及细胞凋亡的影响 ,并探讨其机制。方法　体外培养大鼠主动脉VSMC ,分为不同浓度的Res组及对照组。观察细胞生长曲线 ;四甲基偶氮唑盐 (MTT)微量酶反应法检测细胞活性 ;3H 胸腺嘧啶脱氧核苷 (3H TdR)掺入法观察其对DNA合成的影响 ;HE染色 ,DNA梯带检测及流式细胞术检测细胞凋亡。结果　与对照组比较 ,本研究各浓度Res组VSMC计数均有不同程度的减少 ,3H TdR掺入率明显降低 ,细胞增殖受抑制 ,VSMC凋亡增加 ,并呈浓度依赖性。结论　Res对体外培养的大鼠主动脉VSMC增殖有明显的抑制作用 ,可能是通过抑制细胞DNA合成 ,诱导细胞凋亡等途径发挥作用

Effect of resveratrol on the proliferation of cultured vascular smooth muscle cells

Objectives To observe the effects of resveratrol (Res) on the proliferation of cultured rat vascular smooth muscle cells (VSMC) in vitro,DNA synthesis and apoptosis,and to explore their mechanism.MethodsPrimary culture of VSMCs from aortas of rats was established and Res at different concentrations was added to the medium.The number of VSMCs was counted and the growth curve of cells was recorded. The activity of VSMC was assessed using MTT method and inhibitory rate was calculated too. DNA synthesis was evaluated by 3H]-TdR method and cell apoptosis was examined by DNA laddering,HE stain and flow cytometry.Results Compared with the control group, the number of VSMC decreased,the cell proliferation was inhibited, the rate of 3H]-TdR incorporation declined and cell apoptosis increased in Res groups.These changes presented a dose-dependent manner.Conclusions Res has inhibitory effect on the proliferation of VSMC in vitro,which may result from inhibition of DNA synthesis and accelerating apoptosis.