软脉灵口服液对拟血管性痴呆大鼠空间学习记忆能力及海马CA1区APE/Ref-1表达的影响

目的：观察软脉灵口服液对实验性血管性痴呆（vascular dementia, VaD）大鼠空间学习记忆能力和海马CA1区无嘌呤/无嘧啶核酸内切酶/氧化还原因子1（apurinic/apyrimidinic endonuclease/redox factor-1, APE/Ref-1）表达的影响。 方法：采用双侧颈总动脉永久性结扎制作VaD模型。将45只VaD大鼠随机分成模型组、尼莫地平组、低剂量软脉灵组和高剂量软脉灵组。另外15只正常大鼠行假手术作为对照。于造模次日分别用生理盐水、尼莫地平和软脉灵口服液灌胃，共30 d。用Morris水迷宫实验测定大鼠学习记忆能力，用免疫组织化学法观察大鼠海马CA1区APE/Ref-1的表达。 结果：与模型组比较，高剂量软脉灵组大鼠逃避潜伏期缩短（P〈0.01），1 min穿越原平台所在位置次数增加（P〈0.01）。高剂量软脉灵组与低剂量软脉灵组大鼠海马CA1区APE/Ref-1阳性细胞数比模型组增多（P〈0.01）；与低剂量软脉灵组和尼莫地平组比较，高剂量软脉灵组大鼠海马CA1区APE/Ref-1阳性细胞数增加（P〈0.01），低剂量软脉灵组与尼莫地平组比较，差异无统计学意义。 结论：软脉灵口服液能改善拟VaD大鼠的空间学习记忆能力，对拟VaD大鼠海马CA1区APE/Ref-1表达的下降有抑制作用。

Effects of Ruanmailing Oral Liquid on spatial learning and memory ability and expression of APE/Ref-1 in hippocampal CA1 region in rats with experimental vascular dementia

Objective： To study the effects of Ruanmailing Oral Liquid, a compound traditional Chinese herbal medicine, on spatial learning and memory ability and expression of apurinic/apyrimidinic endonuclease/redox factor-1 （APE/Ref-1） in hippocampal CA1 region in rats with experimental vascular dementia （VaD）.
Methods： VaD was induced in rats by permanent occlusion of bilateral common carotid arteries. Forty-five VaD rats were randomly divided into untreated group, nimodipine group, low-dose Ruanmailing group and high-dose Ruanmailing group. Another 15 rats underwent a sham operation consisting of similar skin incision and manipulation but without occlusion of carotid arteries. From the next day after occlusion, the rats were intragastrically administered with normal saline, nimodipine suspension or Ruanmailing Oral Liquid respectively for 30 days. Morris water maze experiment was adopted to test learning and memory of rats in each group. Expression of APE/Ref-1 protein in the hippocampal CA1 region was measured by immunohistochemical method.
Results： Escape latency was significantly shortened and number of entries in the target area of rats was significantly increased in the high-dose Ruanmailing group as compared with those in the untreated group （P〈0.01）. Compared with the untreated group, count of APE/Ref-1 positive cells was significantly increased in the hippocampal CA1 region in the high- and low-dose Ruanmailing groups （P〈0.01）. Compared with the low-dose group and the nimodipine group, the count of APE/Ref-1 positive cells was remarkably increased in the hippocampal CA1 region in rats of the high-dose Ruanmailing group （P〈0.01）. There was no statistical difference between the low-dose Ruanmailing group and the nimodipine group.
Conclusion： Ruanmailing Oral Liquid can improve the learning and memory ability and enhance the lowered expression level of APE/Ref-1 in the hippocampal CA1 region of rats with VaD.