| HPCE法和HPLC法测定蒲黄中黄酮甙的含量 |
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| 引用本文： | 杨永华,王实强,张水寒. HPCE法和HPLC法测定蒲黄中黄酮甙的含量[J]. 中国中药杂志, 1999, 24(11): 682-684 |
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| 作者姓名： | 杨永华 王实强 张水寒 |
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| 作者单位： | 湖南省中医药研究院 长沙 410006 |
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| 摘 要： | 目的：采用高效毛细管电泳法和高效液相色谱法测定蒲黄中异鼠李素-３-Ｏ-新橙皮糖甙和香蒲新甙的含量。方法：ＨＰＣＥ 用缓冲液０.０２ｍｏｌ·Ｌ－１硼砂０.０５ｍｏｌ·Ｌ－１十二烷基硫酸钠的１０ ％ 乙腈，电压２０ｋＶ，柱温３０ ℃，检测波长２７０ｎｍ ，进样量３０ｋＰａ·ｓ；ＨＰＬＣ 用Ｃ１８ 柱，流动相乙腈水（１５∶８５） ，检测波长２５４ｎｍ 。结果：两种黄酮甙的含量在０－１ ％ ～０－６ ％ 。结论：两种方法均可用于蒲黄的质量控制。
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| 关 键 词： | 蒲黄 HPCE HPLC 含量测定 异鼠李素-3-O-新橙皮糖甙 香蒲新甙 |
| 收稿时间： | 1998-12-07 |
Determination of Flavonoids in Pollen Typhae(Puhuang) by HPCE and HPLC |
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| Yang Yonghua;Wang Shiqiang ; Zhang Shuihan. Determination of Flavonoids in Pollen Typhae(Puhuang) by HPCE and HPLC[J]. China Journal of Chinese Materia Medica, 1999, 24(11): 682-684 |
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| Authors: | Yang Yonghua Wang Shiqiang Zhang Shuihan |
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| Affiliation: | Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410006. |
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| Abstract: | OBJECTIVE: To determine two flavonoids isorhamnetin-3-O-neohesperidoside(I) and typheneoside(II) in Pollen Typhae by HPCE and HPLC. METHOD: HPCE conditions: buffer = 0.02 mol.L-1 borate, 0.05 mol.L-1 SDS and 10% acetonitrile; V = 20 kV; T = 30 degrees C; lambda = 270 nm; sample size = 30 kPa.s, HPLC conditions: C18 lumn, acetonitrile-water (15:85) as mobile phase detection wavelength at 254 nm. RESULT: The contents of flavonoids I and II in five different Typha species and eighteen commercial samples of traditional Chinese drug Puhuang were between 0.1%-0.6%. CONCLUSION: These two methods are rapid, simple, accurate and can be used for the quality control of this traditional Chinese drug. |
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| Keywords: | Pollen Typhae typhaneoside isorhamnetin 3 O neohesperidoside HPCE PHLC |
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