| 中药白芷种质资源的RAPD分析 |
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| 引用本文： | 黄璐琦,王敏,付桂芳,杨滨,王年鹤,袁昌齐. 中药白芷种质资源的RAPD分析[J]. 中国中药杂志, 1999, 24(8): 457-459 |
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| 作者姓名： | 黄璐琦 王敏 付桂芳 杨滨 王年鹤 袁昌齐 |
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| 作者单位： | 中国中医研究院中药研究所 北京 100700;江苏省中国科学院植物研究所 南京 210014 |
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| 基金项目： | 国家中医药管理局青年课题,国家自然科学基金资 |
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| 摘 要： | 目的：对中药白芷的种质资源进行分析。方法：用１２个随机引物对野生白芷、杭白芷的４个居群超过１７个个体的基因组ＤＮＡ进行ＲＡＰＤ分析。结果：共扩增出４０个位点，其中多态位点２６个，占６．５％；祁、杭白芷的多态位点的百分率显示出低水平的遗传变异。根据ＲＡＰＤｉｓｔａｎｃｅ分析，祁、杭间的遗传距离（０．１６０）小于祁白芷居群内单株间和杭白芷不同居群间遗传距离，并且它们都小于野生白芷与祁、杭白芷间的遗传距离。结论：祁、杭白芷应属同一类群，与野生白芷有一定区别。
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| 关 键 词： | 野生白芷 祁白芷 杭白芷 RAPD 种质分析 |
| 收稿时间： | 1998-09-22 |
RAPD Analysis of Germplasm in "Baizhi |
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| Huang Luqi;Wang Min;Fu Guifang ; Yang Bin; Wang Nianhe ; Yuan Changqi. RAPD Analysis of Germplasm in "Baizhi[J]. China Journal of Chinese Materia Medica, 1999, 24(8): 457-459 |
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| Authors: | Huang Luqi Wang Min Fu Guifang Yang Bin Wang Nianhe Yuan Changqi |
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| Affiliation: | Instituute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beijing 100700. |
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| Abstract: | Objective:To analyse the germplasm in Baizhi.Method:Using 12 random primers,over seventeen indivi duals'RAPD were detected in four populations from wild Baizhi (Angelica dahurica) ,Qi Baizhi and Hang Baizhi.Results:A total of 40 loci were amplified,26 of which were polymorphism(65%).The PPS values of Hang Baizhi and Qi Baizhi indicate their low levels of genetic diversity.According to the RAPDinstance analysis,the genetic distance(0.16)between Qi Baizhi and Hang Baizhi is shorter than that of the individuals of the populations of Qi Baizhi and Hng Baizhi,and all of these genetic distances are shorter than those between wild Baizhi and Hang Baizhi(Qi Baizhi).Conclusion:Hang Baizhiand Qi Baizhi belong to the same taxon,which is different from the wild Baizhi. |
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| Keywords: | wild Baizhi( Angelica dahurica ) Qi Baizhi Hang Baizhi RAPD germplasm |
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