鲜地黄与干地黄止血和免疫作用比较研究

目的：从止血和免疫作用方面，比较鲜地黄与干地黄的部分药理作用。方法：①采用阿司匹林造成小鼠凝血时间延长的模型，鲜、干地黄灌胃给药，用毛细玻管法测定凝血时间；②鲜地黄汁或鲜地黄煎液及干地黄煎液给小鼠灌胃给药１０ｄ ，观察对醋酸强的松龙诱导的免疫低下小鼠的腹腔巨噬细胞吞噬功能；③用甲状腺素造成小鼠类阴虚模型，鲜、干地黄灌胃给药，用ＭＴＴ 法测定Ｃｏｎ Ａ 诱导的脾脏淋巴细胞转化；并测定ＬＰＳ 诱导的脾脏淋巴细胞碱性磷酸酶表达活性。结果：鲜地黄汁、鲜地黄煎液和干地黄煎液均在一定程度上拮抗阿司匹林诱导的小鼠凝血时间延长，但鲜地黄汁的作用明显强于干地黄；鲜地黄汁、鲜地黄水煎液能使类阴虚小鼠的脾脏淋巴细胞碱性磷酸酶的表达能力明显增强。鲜地黄汁还可增强ＣｏｎＡ 诱导的脾脏淋巴细胞转化功能。干地黄水煎液对类阴虚小鼠的脾脏Ｂ 淋巴细胞功能也有明显的增强作用，但其作用弱于鲜地黄汁。结论：历史上用鲜地黄汁或鲜地黄水煎液治疗某些疾病（ 如出血性疾病） 有其科学道理，在临床应用上最好遵循之。

A Study on Hemostatic and Immunological Actions of Fresh and Dry Dihuang

Objective:To investigate the difference between fresh and dry Dihuang (Radix Rehmanniae) in hemostatic and immunological actions. Methods:The hemostatic action was assessed in aspirin treated mice;immunological action was assessed by phagocytosis of macrophages in dexamethasone treated mice; and Con A induced mitogenesis and LPS induced alkaline phosphatase activity of splenocytes were determined in thyroxin treated mice.Result:The blood clotting in mice induced by aspirin was inhibited by oral administrations of juice decoction of fresh Dihuang or decoction of dry Dihuang alike. The action of fresh Dihuang juice was stronger than that of dry Dihuang decoction. Oral administrarion of fresh Dihuang juice or decoction for 10 days enhanced LPS induced alkaline phosphatase activity of splenocytes in thyroxin treated mice.The juice also enhanced Con A induced splenocyte mitogenesis.The action of dry Dihuang decoction was weaker than that of fresh juice in increasing LPS induced alkaline phosphatase axtivity.Conclusion:It is recommended to follow the tradition of using fresh Dihuang juice or decoction to treat some diseases such as hemorrhagenic troubles.