| 衍生化气相色谱法测定夏枯草中齐墩果酸和熊果酸的含量 |
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| 引用本文： | 闫花丽,赵陆华,朱丹妮,丁黎. 衍生化气相色谱法测定夏枯草中齐墩果酸和熊果酸的含量[J]. 中国中药杂志, 1999, 24(12): 744-745 |
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| 作者姓名： | 闫花丽 赵陆华 朱丹妮 丁黎 |
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| 作者单位： | 陕西省宝鸡市药品检验所 宝鸡 721001;中国药科大学 南京 210009 |
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| 摘 要： | 目的:气相色谱法测定夏枯草中齐墩果酸和熊果酸的含量。方法:重氮甲烷衍生化气相色谱法,10 % SE-30(2 m ×3 m m) 色谱柱,气化室及检测器温度300 ℃,色谱柱温度27 ℃。结果:线性范围0.0025 ～0 .4mg·ml-1 ,r = 0 .9994 ,齐墩果酸和熊果酸平均回收率分别为93 .53 % ,94 .18 % ,RSD分别为3 .5 % ,3 .0 % 。结论:可用于中药中齐墩果酸和熊果酸成分的分析。
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| 关 键 词： | 夏枯草 齐墩果酸 熊果酸 重氮甲烷衍生化 气相色谱法 |
| 收稿时间： | 1999-01-13 |
Determination of Oleanolic Acid and Ursolic Acid in Spica Prunellae by Derivative GC Method |
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| Yan Huali;Zhao Luhua;Zhu Danni ; Ding Li. Determination of Oleanolic Acid and Ursolic Acid in Spica Prunellae by Derivative GC Method[J]. China Journal of Chinese Materia Medica, 1999, 24(12): 744-745 |
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| Authors: | Yan Huali Zhao Luhua Zhu Danni Ding Li |
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| Affiliation: | Baoji Municipal Institute for Drug Control, Baoji 721001. |
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| Abstract: | OBJECTIVE: To develop a GC method to determine the oleanolic acid and ursolic acid in Spica Prunellae. METHOD: Before GC analysis, the sample was derivatized with CH2N2 solution. The GC conditions were as follows: comumn-10% SE-30(2m x 3mm) and column temperature -270 degrees C. RESULT: The two constituents were well separated and had good linearity in the range of 0.0025-0.4000 mg/ml. The average recoveries and RSD of oleanolic acid were 93.53% and 3.5%, 94.18% and 3.0% respectively. CONCLUSION: The method is good for determining oleanolic acid and ursolic acid in Chinese medicines. |
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| Keywords: | Spica Prunellae oleanolic acid ursolic acid GC CH N derivative |
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