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分光光度法测定刺五加片中总黄酮含量
引用本文:张正康. 分光光度法测定刺五加片中总黄酮含量[J]. 时珍国医国药, 2001, 12(1): 41-42
作者姓名:张正康
作者单位:云南省禄劝县药品检验所
摘    要:目的:建立分光光度法测定刺五加片中总黄酮含量。方法:以芦丁为对照品、硝酸铝-亚硝酸钠为显色剂,于500nm波长处测定吸收度;测定了3个产家5个批号的刺五加片中总黄酮含量。结果:0.002-0.024mg/ml范围线性关系良好(r=0.9925,n=7),加样回收率99.6%(RSD=1.86%);同一产家不同批号及不同产家之间刺五加片中总黄酮含量有明显差异,结论:本法准确可靠,可为控制刺五加片质量提供检测依据。

关 键 词:刺五加片 黄酮 分光光度法 中药 含量
文章编号:1008-0805(2001)01-0041-01
修稿时间:2000-07-18

The Determination forFlavonoids in Tabellae Acanthopanacis Senticosi by UV Spectrophoto-metry Method
ZHANG Zheng kang. The Determination forFlavonoids in Tabellae Acanthopanacis Senticosi by UV Spectrophoto-metry Method[J]. Lishizhen Medicine and Materia Medica Research, 2001, 12(1): 41-42
Authors:ZHANG Zheng kang
Abstract:Objective: UV spectrophotometry method was developed for theflavonoids in Tabellae Acanthopanacis Senticosi. Methods: Rutin was used as comparison, aluminium nitrate-sodium aluminium nitrate was used as color developing agent, at the wavelength of 500 nm. Results: The linear curves was fine between the concentration range 0.002~0.024 mg/ml (r=0.992 5, n=7). The average rate of recovery was 99.6% (RSD=1.86%). Conclusion: This method is accurate.
Keywords:Tabellae Acanthopanacis Senticosi  Flavonoids  UV spectrophotometr  
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